文献类型： 外文期刊

作者： Wang, Zhengwen ¹ ; Li, Xiongxiong ² ; Zhang, Lingyun ¹ ; Wu, Jianping ⁴ ; Zhao, Shengguo ¹ ; Jiao, Ting ² ;

作者机构： 1.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou 730070, Peoples R China

2.Gansu Agr Univ, Key Lab Grassland Ecosyst, Lanzhou 730070, Peoples R China

3.Gansu Agr Univ, Coll Grassland Sci, Lanzhou 730070, Peoples R China

4.Gansu Acad Agr Sci, Lanzhou 730070, Peoples R China

关键词： in vitro; oregano; essential oil; cobalt lactate; fermentation parameters; rumen microorganism

期刊名称：ANIMALS （ 影响因子：3.231； 五年影响因子：3.312 ）

ISSN： 2076-2615

年卷期： 2022 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Simple Summary In the context of a shortage of feed resources and a complete ban on veterinary antibiotics, searching for green additives that can improve the production performance of ruminants has become a popular research topic. Oregano essential oil (EO) inhibits rumen gas production (GP) and regulates animal digestive metabolism, and cobalt lactate (Co) can improve feed digestibility. However, previous studies on EO of oregano and Co showed different results. Therefore, the present study aimed to investigate the effects of different EOC addition levels on rumen in vitro fermentation and rumen bacterial community composition, and the experimental data obtained showed that all five EOC (0.1425% cobalt lactate + 1.13% oregano essential oil + 98.7275% carrier) addition levels in this experiment had no significant effect on nutrient digestibility. However, the addition of 1500 mg center dot L-1 EOC significantly improved rumen fermentation parameters and altered the microbiota composition. All presented data provide a theoretical basis for the application of oregano essential oil and cobalt in ruminant nutrition. The objective of this experiment was to evaluate the effect of different EOC (0.1425% cobalt lactate + 1.13% oregano essential oil + 98.7275% carrier) levels on in vitro rumen fermentation and microbial changes. Six EOC levels (treatments: 0 mg center dot L-1, CON; 50 mg center dot L-1, EOC1; 100 mg center dot L-1, EOC2; 400 mg center dot L-1, EOC3; 800 mg center dot L-1, EOC4 and 1500 mg center dot L-1, EOC5) were selected to be used to in vitro incubation. The in vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD), in vitro acid detergent fiber digestibility (IVNDFD), pH, ammonia-nitrogen (NH3-N) concentration, total volatile fatty acid (TVFA) concentration and microbial protein (MCP) concentration were measured after 48 h incubation, after which the groups with significant nutrient digestibility and fermentation parameters were subjected to 16S rRNA sequencing. The results showed that the total gas production (GP) of the EOC5 group was higher than that of the other groups after 12 h of in vitro incubation. TVFA, NH3-N and MCP concentrations were also shown to be higher in group EOC5 than those in other groups (p < 0.05), while NH3-N and MCP concentrations in the EOC2 group were lower than those in other groups significantly (p < 0.05). The molar ratio of acetic acid decreased while the molar ratio of propionic acid increased after the addition of EOC. 16S rRNA sequencing revealed that the rumen microbiota was altered in response to adding EOC, especially for the EOC5 treatment, with firmicutes shown to be the most abundant (43.1%). The relative abundance of Rikenellaceae_RC9_gut_group was significantly lower, while the relative abundance of uncultured_bacterium_f_Muribaculaceae and Succiniclasticum was significantly higher in the EOC5 group than those in other groups (p < 0.05). Comprehensive analysis showed that EOC (1500 mg center dot L-1) could significantly increase gas production, alter sheep rumen fermentation parameters and microbiota composition.