Systematic Analysis of the Maize OSCA Genes Revealing ZmOSCA Family Members Involved in Osmotic Stress and ZmOSCA2.4 Confers Enhanced Drought Tolerance in Transgenic Arabidopsis
文献类型： 外文期刊
作者： Cao, Liru 1 ; Zhang, Pengyu 1 ; Lu, Xiaomin 2 ; Wang, Guorui 1 ; Wang, Zhenhua 2 ; Zhang, Qianjin 2 ; Zhang, Xin 2 ; Wei, 1 ;
作者机构： 1.Henan Agr Univ, Natl Key Lab Wheat & Maize Crop Sci, Coll Agron, Zhengzhou 450002, Henan, Peoples R China
2.Henan Acad Agr Sci, Grain Crops Res Inst, Zhengzhou 450002, Henan, Peoples R China
3.Natl Engn Res Ctr Wheat, Zhengzhou 450002, Henan, Peoples R China
关键词： OSCAs; co-expression modules; proline content; DUF221 domain; abiotic stresses; interaction; drought resistance
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2020 年 21 卷 1 期
页码：
收录情况： SCI
摘要： OSCAs are hyperosmolality-gated calcium-permeable channel proteins. In this study, two co-expression modules, which are strongly associated with maize proline content, were screened by weighted correlation network analysis, including three ZmOSCA family members. Phylogenetic and protein domain analyses revealed that 12 ZmOSCA members were classified into four classes, which all contained DUF221 domain. The promoter region contained multiple core elements responsive to abiotic stresses and hormones. Colinear analysis revealed that ZmOSCAs had diversified prior to maize divergence. Most ZmOSCAs responded positively to ABA, PEG, and NaCl treatments. ZmOSCA2.3 and ZmOSCA2.4 were up-regulated by more than 200-fold under the three stresses, and showed significant positive correlations with proline content. Yeast two-hybrid and bimolecular fluorescence complementation indicated that ZmOSCA2.3 and ZmOSCA2.4 proteins interacted with ZmEREB198. Over-expression of ZmOSCA2.4 in Arabidopsis remarkably improved drought resistance. Moreover, over-expression of ZmOSCA2.4 enhanced the expression of drought tolerance-associated genes and reduced the expression of senescence-associated genes. We also found that perhaps ZmOSCA2.4 was regulated by miR5054.The results provide a high-quality molecular resource for selecting resistant breeding, and lay a foundation for elucidating regulatory mechanism of ZmOSCA under abiotic stresses.
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