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Molecular characterization of the myostatin gene and its regulation on muscle growth in Yesso scallop Patinopecten yessoensis

文献类型： 外文期刊

作者： Sun, Xiujun 1 ; Li, Li 3 ; Liu, Zhihong 1 ; Zhao, Dan 1 ; Yang, Aiguo 1 ; Zhou, Liqing 1 ; Wu, Biao 1 ; Tian, Jiteng 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Qingdao 266071, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266200, Peoples R China

3.Marine Biol Inst Shandong Prov, Qingdao 266104, Peoples R China

4.Shanghai Ocean Univ, Shanghai 201306, Peoples R China

关键词： Scallops; Myostatin; Adductor muscle; Whole-mount in situ hybridization; RNA interference

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2020 年 520 卷

页码：

收录情况： SCI

摘要： Myostatin (MSTN) belongs to the transforming growth factor beta (TGF-beta) family, which plays a critical role in negatively regulating muscle growth in vertebrates. However, little is known about its function in muscle growth of scallops that possess a large adductor muscle composed of striated and smooth muscle fibers. In this study, we first indentify and characterize the complete cDNA sequence of the myostatin gene from Yesso scallop P. yessoensis, an economically important species in Asian countries. The C-terminal mature TGF-beta peptides are highly conserved in scallops, with sequence identity of > 97% among these species. Gene expression at developmental stages revealed by whole-mount in situ hybridization indicates that myostatin is mainly expressed on the profusely ciliated rim of velum in veligers. The significant increase of muscle cellularity induced by RNA interference of myostatin can be explained as a result of a combination of hyperplasia and hypertrophy of MHC II striated myofibers, rather than myofiber growth of the paramyosin-rich smooth muscle. This is also evidenced by the quantification results of muscle gene expression, which indicate the significant increase of MHC expression after the inhibition of myostatin mRNA in the striated muscle, but not in the smooth muscle. For both of the striated and smooth muscles, however, no significant difference between the treatment and control groups is detected by real-time quantitative PCR analysis in other muscle genes. These findings suggest that the cellular targets of myostatin in scallop muscles are probably regulated by signaling directly to striated MHC. The new additional nuclei are potentially supplied by a population of satellite cells or muscle stem cell-like adult muscle precursors that may fuse with existing muscle fibers during muscle hypertrophy. These evidences found in this study highlight that myostatin may not only be involved in the development of larval musculature, but also play an important role in regulating scallop muscle growth.

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