Overexpression of a samphire high-affinity potassium transporter gene SbHKT1 enhances salt tolerance in transgenic cotton
文献类型： 外文期刊
作者： Guo, Qi 1 ; Meng, Shan 1 ; Tao, Shucui 1 ; Feng, Juan 1 ; Fan, Xinqi 1 ; Xu, Peng 1 ; Xu, Zhenzhen 1 ; Shen, Xinlian 1 ;
作者机构： 1.JAAS, Inst Ind Crops, Minist Agr & Rural Affairs, Key Lab Cotton & Rapeseed Nanjing, Nanjing 210014, Peoples R China
关键词： Cotton; Overexpression; Salt stress; SbHKT1
期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）
ISSN： 0137-5881
年卷期： 2020 年 42 卷 3 期
页码：
收录情况： SCI
摘要： Under salt stress, the plants need to maintain a low Na+ concentration and Na+/K+ ratio in the cell cytoplasm to keep normal growth and development. High-affinity K+ transporter (HKT) genes are known to play an important role in regulating the transportation of Na+ and K+ in higher plants. However, reports on its potential role in conferring stress tolerance in cotton are rare. In a previous study, we isolated a potassium transporter SbHKT1 from halophyte Salicornia bigelovii. With the intention to assess whether the SbHKT1 gene would improve salt tolerance in cotton, cotton plants overexpressing SbHKT1 were generated by Agrobacterium-mediated transgenic technology. Overexpression of SbHKT1 in cotton increased germination rate and biomass as well as root systems compared with wild-type plants. Transgenic cotton had significantly higher K+ content, lower Na+ content, and lower Na+/K+ ratio than wild-type plants in leaves, stems and roots under salt stress. Moreover, there were significant higher activity of antioxidant enzymes including SOD, POD, and CAT and lower malondialdehyde content which means better cell membrane integrity in transgenic cotton compared to control plants. These results indicated that overexpressing SbHKT1 in cotton improved salt tolerance by increasing the capacity of K+ uptake, K+/Na+ homeostasis, and the scavenging of reactive oxygen species.
- 相关文献
作者其他论文 更多>>
-
Unveiling the Role of GhP5CS1 in Cotton Salt Stress Tolerance: A Comprehensive Genomic and Functional Analysis of P5CS Genes
作者：Fang, Hui;Gao, Xin;Wu, Yunhao;Zhang, Ke;Wu, Ying;Li, Junyi;Qian, Dongmei;Li, Ruochen;Gu, Haijing;Mehari, Teame Gereziher;Wang, Baohua;Mehari, Teame Gereziher;Shen, Xinlian
关键词：cotton genomics;
P5CS gene family;GhP5CS1 function; salt stress; proline; virus-induced gene silencing (VIGS) -
Development of a set of monosomic alien addition lines from Gossypium raimondii in Gossypium hirsutum toward breeding applications in cotton
作者：Xu, Zhenzhen;Lu, Xiaoxi;Ding, Wenjie;Ji, Wei;Ali, Wuzhimu;Wang, Heyang;Meng, Shan;Guo, Qi;Xu, Peng;Chen, Xianglong;Zhao, Liang;Yu, Huan;Shen, Xinlian
关键词：
Gossypium hirsutum ;Gossypium raimondii ; Monosomic alien addition lines; Fluorescence in situ hybridization (FISH); SSR marker analysis -
Verticillium dahliae effector Vd06254 disrupts cotton defence response by interfering with GhMYC3-GhCCD8-mediated hormonal crosstalk between jasmonic acid and strigolactones
作者：Ma, Jianhui;Jiang, Fan;Yu, Yan;Zhou, Haodan;Zhan, Jingjing;Li, Jianing;Chen, Yanli;Wang, Ye;Duan, Hongying;Ge, Xiaoyang;Zhao, Hang;Liu, Lisen;Zhou, Haodan;Zhan, Jingjing;Li, Jianing;Chen, Yanli;Wang, Ye;Ge, Xiaoyang;Zhao, Hang;Liu, Lisen;Xu, Zhenzhen;Zhao, Hang
关键词：
Verticillium dahliae ; Effector; host-pathogen interaction; cotton; transcription factor -
Centromere-size reduction and chromatin state dynamics following intergenomic hybridization in cotton
作者：Han, Jinlei;Dai, Yan;Zhang, Xin;Tian, Jingjing;Zhou, Jialiang;Xu, Xinqi;Chen, Qi;Kou, Xiaobing;Xu, Lei;Wu, Xinyu;Sun, Ziying;Geng, Jiahui;Li, Lin;Qiu, Chenyu;Mehari, Teame Gereziher;Wang, Baohua;Zhang, Hui;Wang, Kai;Hu, Guanjing;Wendel, Jonathan F.;Hu, Guanjing;Shen, Xinlian;Xu, Zhenzhen
关键词：
-
Herbicide resistance in Leptochloa chinensis (L.) Nees populations from different regions of Jiangsu Province, China: sensitivity differences and underlying mechanisms
作者：Xu, Peng;Ju, Yawen;Fu, Yousheng;Cao, Kaige;Wang, Ke;Wang, Hongchun;Zhu, Axiu
关键词：
Leptochloa chinensis (L.) Nees; geographical populations; herbicide; cyhalofop-butyl; sensitivity; target mutation -
Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis
作者：Zhang, Hongmei;Zhang, Wei;Liu, Xiaoqing;Chen, Xin;Chen, Huatao;Meng, Shan;Yan, Wei;Hui, Linchong;Chen, Wei
关键词：
-
Transcriptomic profiling reveals salt-responsive long non-coding RNAs and their putative target genes for improving salt tolerance in upland cotton (Gossypium hirsutum)
作者：Feng, Wenxiang;Fang, Hui;Mehari, Teame Gereziher;Gu, Haijing;Wu, Ying;Jia, Mengxue;Han, Jinlei;Wang, Kai;Wang, Baohua;Guo, Lishuang;Li, Feng;Chen, Haodong;Guo, Qi;Xu, Zhenzhen;Shen, Xinlian;Ditta, Allah;Khan, Muhammad K. R.
关键词：Gossypium hirsutum; Transcriptome; LncRNA; Salt stress; VIGS
