Development of polyclonal-antibody-coated immunomagnetic beads for separation and detection of koi herpesvirus in large-volume samples
文献类型： 外文期刊
作者： Zhang, Min 1 ; Li, Yingying 2 ; Jing, Hongli 1 ; Wang, Na 1 ; Wu, Shaoqiang 1 ; Wang, Qing 2 ; Lin, Xiangmei 1 ;
作者机构： 1.Chinese Acad Inspect & Quarantine, Inst Anim Quarantine, 11 Bldg,Ronghua South Rd, Beijing 100176, Peoples R China
2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol Guangdong Prov, Key Lab Fishery Drug Dev,Minist Agr & Rural Affai, Guangzhou 510380, Guangdong, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2020 年 165 卷 4 期
页码：
收录情况： SCI
摘要： To separate and concentrate koi herpesvirus (KHV) from large-volume samples, a separation method based on immunomagnetic beads (IMBs) coated with polyclonal antibody directed against KHV was developed. After treatment with IMBs, viral DNA was extracted from samples and used as a template for quantitative PCR (qPCR). The results showed that the concentration of the template DNA extracted from the virus that had been separated using IMBs was 9.65-fold higher than that from virus not treated with IMBs. The detection limit of the IMBs/qPCR method was found to be at least 10 times lower than that of qPCR alone.
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