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Multiplex RT-PCR Assay for the Simultaneous Detection and Identification of Five Sugarcane Viruses

文献类型： 外文期刊

作者： Feng, Xiao-Yan 1 ; Wang, Wen-Zhi 1 ; Shen, Lin-Bo 1 ; Wang, Jun-Gang 1 ; Xiong, Guo-Ru 1 ; Feng, Cui-Lian 1 ; Zhao, Ti 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, 4 Coll Rd, Haikou 571101, Hainan, Peoples R China

关键词： Multiplex RT-PCR; Simultaneous detection; Sugarcane; Virus

期刊名称：SUGAR TECH （ 影响因子：1.591； 五年影响因子：1.688 ）

ISSN： 0972-1525

年卷期：

页码：

收录情况： SCI

摘要： Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Sugarcane streak mosaic virus (SCSMV), Sugarcane yellow leaf virus (SCYLV), and Sugarcane bacilliform virus (SCBV) are the major viruses infecting sugarcane worldwide. This study proposed a multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay for the simultaneous detection and identification of these sugarcane viruses. Results showed that five primer pairs amplified their respective target viruses, indicating the assay specificity. The sensitivity of the assay was similar to or 10-100-folds lower than that of singleplex RT-PCR for detecting SCMV or the four other viruses, respectively. When detecting the five viruses in field sugarcane samples, the detection results of multiplex RT-PCR were approximately identical to those of singleplex RT-PCR, thereby indicating the relative reliability of the former. The proposed multiplex RT-PCR can simultaneously detect SCMV, SrMV, SCSMV, SCYLV, and SCBV in sugarcane, making it a suitable, efficient, and practical detection method for sugarcane viruses.

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