Carbon nanotube-based DNA vaccine against koi herpesvirus given by intramuscular injection
文献类型： 外文期刊
作者： Hu, Feng 1 ; Li, Yingying 1 ; Wang, Qing 1 ; Wang, Gaoxue 3 ; Zhu, Bin 3 ; Wang, Yingying 1 ; Zeng, Weiwei 1 ; Yin, Jiyua 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev,Minist Agr & Rural Affai, Key Lab Aquat Anim Immune Technol Guangdong Prov, Guangzhou, Guangdong, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
3.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China
4.FLI, Inst Infectol, Fed Res Inst Anim Hlth, German Reference Lab KHVD, Greifswald, Germany
关键词： Koi herpes virus; ORF149; Carbon nanotubes; Immune response; DNA vaccine
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2020 年 98 卷
页码：
收录情况： SCI
摘要： Koi herpesvirus (KHV) also named Cyprinid Herpesvirus 3 (CyHV-3) is one of the most threatening pathogens affecting common carp production as well as the valued ornamental koi carp. The current commercial vaccines available are costly and potentially cause severe stress caused by live virus. KHV ORF149 gene has been proved encoding one of the main immunogenic proteins for KHV. In this study, we coupled a plasmid expression vector for ORF149 to single walled carbon nanotubes (SWCNTs) for an anti-KHV vaccine. The vaccine conferred an 81.9% protection against intraperitoneal challenge with KHV. Importantly, SWCNTs as a promising vehicle can enhanced the protective effects 33.9% over that of the naked DNA vaccine at the same dose. The protection was longer and serum antibody production, enzyme activities and immune-related gene expression were all induced in fish vaccinated with the nanotube-DNA vaccine compared with the DNA alone. Thereby, this study demonstrates that the ORF149 DNA vaccine loaded onto SWCNTs as a novel vaccine might provide an effective method of coping with KHV disease using intra-muscular vaccination.
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