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An effective live attenuated vaccine against Streptococcus agalactiae infection in farmed Nile tilapia (Oreochromis niloticus)

文献类型： 外文期刊

作者： Zhang, Defeng 1 ; Gao, Yanxia 1 ; Li, Qingyong 2 ; Ke, Xiaoli 1 ; Liu, Zhigang 1 ; Lu, Maixin 1 ; Shi, Cunbin 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr & Rural Affairs, Guangzhou 510380, Peoples R China

2.Fisheries Res & Extens Ctr Huizhou, Huizhou 516002, Peoples R China

3.Chinese Acad Fishery Sci, Key Lab Aquat Anim Immune Technol, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China

关键词： Streptococcus agalactiae; Live attenuated vaccine; Capsular polysaccharide; Immunoprotection; Tilapia

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2020 年 98 卷

页码：

收录情况： SCI

摘要： Streptococcus agalactiae is an important pathogen associated with various aquatic animals, especially tilapia. Streptococcosis has greatly limited the healthy development of tilapia aquaculture in recent times. The development of novel effective vaccines is important for the prevention and control of streptococcosis in fish. We previously constructed a non-encapsulated S. agalactiae strain Delta cps by the in-frame deletion method. Here, we evaluated whether this mutant Delta cps is safe for tilapia and suitable for protection against streptococcosis. We observed that the Delta cps strain was non-pathogenic to tilapia, and there was no reversion of virulence when it was passaged in tilapia. Moreover, the Delta cps strain survived for at least 11 d in the main immune organs of tilapia. The tilapia vaccinated via intraperitoneal (IP) injection with Delta cps strain induced a high antibody titer, and the IgM antibody levels were significantly higher in the vaccinated group than in the control group. The vaccination protected tilapia against the S. agalactiae challenge with a relative percent survival of 90.47%. In addition, tilapia immunized with the /cps strain showed significantly higher expression level of IFN-gamma, IL-1 beta, MyD88, IgM, and MHC-I alpha in the head kidney than those in the control during the entire observation period. The expression of MHC-143 was inhibited during 1-7 d of immunization. These results revealed that the Delta cps strain is able to induce humoral and cell-mediated immune response in tilapia. Therefore, the strain Delta cps has a broad application prospect as a target for attenuation in vaccine development.

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