Comprehensive expression analysis of the beta integrin from Penaeus monodon indicating its participation in innate immunity and ammonia nitrogen stress response
文献类型： 外文期刊
作者： Shi, Mengke 1 ; Jiang, Shigui 1 ; Li, Yundong 1 ; Yang, Qibin 1 ; Jiang, Song 1 ; Yang, Lishi 1 ; Huang, Jianhua 1 ; Zhou 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Minist Agr, Guangzhou 510300, Peoples R China
2.Shanghai Ocean Univ, Coll Aqua Life Sci & Technol, Shanghai, Peoples R China
3.Chinese Acad Fishery Sci, Shenzhen Base South China Sea Fisheries Res Inst, Shenzhen 518108, Peoples R China
关键词： Penaeus monodon; Beta integrin subunit; dsRNA; Innate immunity; Ammonia nitrogen stress; Apoptosis
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2020 年 98 卷
页码：
收录情况： SCI
摘要： The aim of the present study was to investigate the function of the beta integrin (PmItgb) in Penaeus monodon. The 3011 bp cDNA sequence of PmItgb was cloned from P. monodon using rapid amplification of cDNA ends (RACE) PCR. Phylogenetic tree analyses indicated that the amino acid sequence of PmItgb should be merged into Fenneropenaeus chinensis (93%). Quantitative real-time PCR (q RT-PCR) revealed that PmItgb mRNA was highly expressed in the hemocytes. In addition, with regard to developmental stages, Pmltgb showed significantly higher expression in oosperm, nauplius IV, zoea I and III, and post larval stages than that in other development stages. Pmltgb expression in the shrimp epidermis was higher in the postmolt (B) stage, and lower in other molting stages. We also found that Vibrio harveyi and V. anguillarum challenge enhanced Pmltgb expression in the hepatopancreas and gills. When Pmltgb was inhibited, innate immunity-related genes such as ALF, crustin 1, crustin 7, penaeidin 3, and penaeidin 5 were significantly down-regulated. Furthermore, we demonstrated that PmItgb knock-down by specific dsRNA reduced bacterial clearance. In high ammonia nitrogen concentrations, Pmltgb was significantly up-regulated in the hepatopancreas and gills. After PmItgb was silenced, the rate of mortality owing to high ammonia nitrogen concentrations decreased; the expression of related anti-apoptotic genes was up-regulated, and that of the apoptotic genes was slightly down-regulated. These results suggested that PmItgb may be involved in shrimp innate immunity and mediate apoptosis of hepatopancreatic cells induced by high ammonia nitrogen environments.
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