Toll-like receptor (TLR) 2 and TLR13 from the endangered primitive-ray finned fish Dabry's sturgeon (Acipenser dabryanus) and their expression profiling upon immune stimulation
文献类型： 外文期刊
作者： Tang, Rong 1 ; Wang, Sisi 1 ; Han, Panpan 3 ; Zhang, Qihuan 1 ; Zhang, Shuhuan 4 ; Xing, Xiaoping 1 ; Shao, Rong 1 ; Xu, 1 ;
作者机构： 1.Yancheng Inst Technol, Jiangsu Key Lab Biochem & Biotechnol Marine Wetla, Yancheng 224051, Jiangsu, Peoples R China
2.Jiangsu Univ Sci & Technol, Coll Biotechnol, Zhenjiang 212003, Jiangsu, Peoples R China
3.Yangtze Univ, Coll Anim Sci, Jingzhou 434020, Hubei, Peoples R China
4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Wuhan 430223, Hubei, Peoples R China
关键词： Acipenser dabryanus; Toll-like receptors; Gene clone; LPS; PolyI:C
期刊名称：AQUACULTURE REPORTS （ 影响因子：3.216； 五年影响因子：3.812 ）
ISSN： 2352-5134
年卷期： 2020 年 16 卷
页码：
收录情况： SCI
摘要： Toll-like receptors (TLRs) are important pattern recognition receptors (PRRs) which can recognize a series of pathogen associated molecular patterns (PAMPs) to initiate the host innate immune responses. In the present study, two TLRs (TLR2 and TLR13) were identified for the first time from the endangered primitive-ray finned fish Dabry's sturgeon (Acipenser dabryanus), a critically endangered species. The full-length of sturgeon TLR2 was 2591 bp, including an open reading frame (ORF) of 2394 bp which encoded a polypeptide of 797 amino acids. The full-length adaTLR13 cDNA was 3648 bp, consisting of an ORF of 2889 bp which encoded a polypeptide of 962 amino acids. Both TLRs shared high sequence identity/similarity with their counterparts from other fish species. In addition, both TLRs contained typical TLRs motifs, including a signal peptide, eight LRRs, an LRR-CT domain in the extracellular N-terminal, a transmembrane domain, and a C-terminal intracellular TIR domain. Realtime qPCR assays showed that both were ubiquitously detected in all examined tissues, but exerted different expression patterns. Following LPS and polyl:C stimulation, both TLRs were up-regulated in head-kidney primary leucocytes, indicating that both TLRs might involve in recognizing LPS and polyI:C. To the best of our knowledge, this is the first report about the gene structure and expression of TLR2 and TLR13 in Dabry's sturgeon. Our results enriched the repertoire of TLRs of Dabry's sturgeon and provided the basis for understanding the functional evolutionary history of vertebrates' TLRs.
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