A universal pipeline for mobile mRNA detection and insights into heterografting advantages under chilling stress
文献类型： 外文期刊
作者： Wang, Ying 1 ; Wang, Lingping 1 ; Xing, Nailin 2 ; Wu, Xiaohua 1 ; Wu, Xinyi 1 ; Wang, Baogen 1 ; Lu, Zhongfu 1 ; Xu, Pei 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Peoples R China
2.Ningbo Acad Agr Sci, Inst Vegetables, Ningbo 315040, Peoples R China
3.Zhejiang Acad Agr Sci, State Key Lab Qual & Safety Agroprod, Shanghai 201800, Peoples R China
4.Biozeron Biotechnol Co Ltd, Shanghai 201800, Peoples R China
5.China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：6.793； 五年影响因子：6.589 ）
ISSN： 2662-6810
年卷期： 2020 年 7 卷 1 期
页码：
收录情况： SCI
摘要： Heterografting has long been used to enhance the chilling tolerance of temperature-sensitive crops, including watermelon, whose mechanism is known to involve bidirectional long-distance mRNA movements. Despite several studies reporting on mobile mRNA (mb-mRNA) profiles in plants, accurate identification of mb-mRNAs is challenging owing to an array of technical problems. Here, we developed a bioinformatical pipeline that took most of the known technical concerns into consideration and is considered to be a universal tool for mb-mRNA detection in heterografts. By applying this pipeline to a commercial watermelon-bottle gourd heterografting system, we detected 130 and 1144 mb-mRNAs upwardly and 167 and 1051 mb-mRNAs downwardly transmitted under normal and chilling-stress conditions, respectively. Quantitative real-time PCR indicated a high accuracy rate (88.2%) of mb-mRNA prediction with our pipeline. We further revealed that the mobility of mRNAs was not associated with their abundance. Functional annotation and classification implied that scions may convey the stress signal to the rootstock, subsequently triggering energy metabolism reprogramming and abscisic acid-mediated stress responses by upward movement of effective mRNAs, ultimately leading to enhanced chilling tolerance. This study provides a universal tool for mb-mRNA detection in plant heterografting systems and novel insights into heterografting advantages under chilling stress.
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