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Analysis of ginseng root and leaf by multiple columns and detections liquid chromatography

文献类型： 外文期刊

作者： Qian, Zhengming 1 ; Huang, Qi 2 ; Li, Chunhong 2 ; Chen, Jing 1 ; Li, Gang 3 ; Ma, Hongyan 4 ; Xie, Juying 1 ;

作者机构： 1.Xiangnan Univ, Sch Rehabil, Chenzhou 423000, Peoples R China

2.Sunshine Lake Pharma Co LTD, Key Lab State Adm Tradit Chinese Med, Dongguan, Peoples R China

3.Jilin Acad Agr Sci, Agr Environm & Resources Res Ctr, Changchun, Peoples R China

4.Guangdong Pharmaceut Univ, Sch Tradit Chinese Med, Guangzhou 510006, Peoples R China

关键词： Ginseng leaf; ginseng root; HPLC; multiple columns; multiple detections

期刊名称：JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES （ 影响因子：1.312； 五年影响因子：1.145 ）

ISSN： 1082-6076

年卷期：

页码：

收录情况： SCI

摘要： A multiple columns and detections liquid chromatography system, including size exclusion chromatography (SEC) and reversed phase liquid chromatography (RPLC), for the analysis of macromolecules and micromolecules in ginseng root and leaf was developed. The columns were connected by two switching valves. Macromolecules were separated on a SEC column (TSK gel SuperMultipore PW-H column, 6 mmx 150 mm, 8 mu m) by isocratic elution of 50 mM ammonium acetate aqueous solution, 0.3 mL/min of flow rate and detected by evaporative light scattering detection (ELSD). Micromolecules were analyzed on a Poroshell RP column (Agilent Poroshell 120 SB-Aq column, 4.6 mm x 50 mm, 2.7 mu m) with gradient elution of water and acetonitrile, 0.6 mL/min of flow rate and detected by ultraviolet detection (UV). As a result, in the macromolecules chromatogram of ginseng root sample showed two main peaks while only one major peak for ginseng leaf. For micromolecules analysis, 17 compounds (3 nucleosides + 14 saponins) and 17 compounds (3 nucleosides + 1 flavonoid + 13 saponins) were found in ginseng root and leaf, respectively. The developed method is helpful for the quality evaluation of ginseng root and leaf.

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