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Multiple down-regulated cytochrome P450 monooxygenase genes contributed to synergistic interaction between chlorpyrifos and imidacloprid against Nilaparvata lugens

文献类型： 外文期刊

作者： Xu, Lu 1 ; Luo, Guanghua 1 ; Sun, Yang 4 ; Huang, Shuijin 4 ; Xu, Dejin 1 ; Xu, Guangchun 1 ; Han, Zhaojun 3 ; Gu, Zhongy 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base, Nanjing 210014, Peoples R China

2.Huaibei Normal Univ, Coll Life Sci, Huaibei 235000, Peoples R China

3.Nanjing Agr Univ, Coll Plant Protect, Educ Minist, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Peoples R China

4.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanchang 330200, Jiangxi, Peoples R China

关键词： Nilaparvata lugens; Transcriptome; Synergism; Chlorpyrifos and imidacloprid mixture; Cytochrome P450 monooxygenase; RNAi

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2020 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Insecticide mixtures are an effective strategy in pest resistance management. The synergistic chlorpyrifos and imidacloprid mixture could significantly increase toxicity against rice pest, Nilaparvata lugens, despite their high levels of resistance. However, synergism mechanisms to explain this phenomenon remain unknown. Chlorpyrifos and imidacloprid at a 1:0.5 ratio showed significant synergism on N. lugens with a combination index value of 0.18 after topical exposure. We constructed a genetic database of the genes expressed in individual and synergistic chlorpyrifos and imidacloprid treatments of N. lugens using Illumina Hiseq (TM) X Ten, and 17 co-downregulated genes putatively involved in synergism were detected by comparative transcriptome analyses. Expression patterns of the 17 candidate synergistic genes matched with transcriptome sequencing data by quantitative real-time PCR analyses. Feeding of dsRNAs further reduced the expression levels of 10 of these candidate synergistic genes (from 1.68 to 4.13-fold). Nymphs fed with only dsRNAs of CYP4DE1, CYP6AY1v2, CYP353D1, and CYP439A1 experienced more high mortality rates (81.45-90.34%) to improve synergism between chlorpyrifos and imidacloprid. Multiple reductive expressed P450 genes were potentially associated with synergism of a mixture of chlorpyrifos and imidacloprid, as confirmed by comparative transcriptome analyses and RNAi assays. Our findings suggested that synergistic interactions between chlorpyrifos and imidacloprid might be controlled by P450s.

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