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Fed-batch high-solids enzymatic saccharification of lignocellulosic substrates with a combination of additives and accessory enzymes

文献类型： 外文期刊

作者： Mukasekuru, Marie Rose 1 ; Kaneza, Pascal 1 ; Sun, Haiyan 3 ; Sun, Fubao Fuelbiol 1 ; He, Jing 4 ; Zheng, Pu 1 ;

作者机构： 1.Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Carbohydrate Chem & Biotechnol, Wuxi 214122, Jiangsu, Peoples R China

2.Coll Sci & Technol, Dept Biol, Ave Armee,POB 3900, Kigali, Rwanda

3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

4.Minist Agr, Key Lab Dev & Applicat Rural Renewable Energy, Biogas Inst, Chengdu 610041, Peoples R China

5.Natl Agr Sci & Technol Ctr, Chengdu 610041, Peoples R China

关键词： Sugarcane bagasse; Alkaline-catalyzed glycerol organosolv (al-AGO) pretreatment; Additives and accessory enzymes; High-solids enzymatic saccharification; Low enzyme loading; Fed-batch mode

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.645； 五年影响因子：5.749 ）

ISSN： 0926-6690

年卷期： 2020 年 146 卷

页码：

收录情况： SCI

摘要： The operation of high-solids enzymatic saccharification at low cellulase loading is required for the commercialization of the current cellulase-mediated lignocellulosic bioethanol. In this study, high-solids (30 %) fed-batch enzymatic saccharification at low cellulase loading (3 FPU/g) was attempted by fed-batch mode with a mixture of additives and accessory enzymes. The saccharification process began with 10 % (w/v) solids content and 6 %, 6 %, and 8 % of substrates were fed at 6, 12, and 24 h to achieve 30 % (w/v) of the total solids content. With the optimization of additives, namely 40 mg/g Tween 80, 30 mg/g tea saponin, 8 mg/g BSA, and 2.4 mg/g xylanase and 0.4 mg/g AA9 as accessory enzymes, the saccharification process released 180 g/L of fermentable sugars with 70 % glucose yield after 72 h. The fed-batch mode along with the mixtures of some additives and accessory enzymes has been found to be a beneficial approach to run a high-solids enzymatic saccharification at low enzyme loading.

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