An array of 60,000 antibodies for proteome-scale antibody generation and target discovery
文献类型： 外文期刊
作者： Wang, Zhaohui 1 ; Li, Yang 2 ; Hou, Bing 1 ; Pronobis, Mira, I 4 ; Wang, Mingqiao 3 ; Wang, Yuemeng 3 ; Cheng, Guangcu 1 ;
作者机构： 1.Northwest Univ, Sch Life Sci, Xian 710069, Shanxi, Peoples R China
2.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai 200240, Peoples R China
3.Abmart, 333 Guiping Rd, Shanghai 200033, Peoples R China
4.Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA
5.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
6.Minist Agr, Guangxi Key Lab Sugarcane Genet Improvement, Key Lab Sugarcane Biotechnol & Genet Improvement, Nanning 530007, Guangxi, Peoples R China
7.China Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod, Minist Agr, Beijing 100193, Peoples R China
8.China Agr Univ, Coll Anim Sci & Technol, Natl Engn Lab Anim Breeding, Beijing 100193, Peoples R China
9.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Zhengzhou 450009, Henan, Peoples R China
10.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100083, Peoples R China
11.Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400716, Peoples R China
12.China Japan Friendship Hosp, Ctr Resp Med, Natl Clin Res Ctr Resp Dis, Dept Pulm & Crit Care Med, Beijing 100029, Peoples R China
13.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China
14.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China
15.Ludwig Inst Canc Res, 9500 Gilman Dr, La Jolla, CA 92093 USA
16.Karolinska Inst, Dept Med Biochem & Biophys, Div Funct Genom & Syst Biol, S-17165 Stockholm, Sweden
17.South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China
18.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China
19.South China Agr Univ, Key Lab Chicken Genet Breeding & Reprod, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China
20.Wuhan Univ, Inst Adv Studies, Wuhan, Peoples R China
21.Wuhan Univ, Coll Life Sci, Wuhan, Peoples R China
22.State Key Lab Oncogenes & Related Genes, Shanghai 200240, Peoples R China
期刊名称：SCIENCE ADVANCES （ 影响因子：14.136； 五年影响因子：16.446 ）
ISSN： 2375-2548
年卷期： 2020 年 6 卷 11 期
页码：
收录情况： SCI
摘要： Antibodies are essential for elucidating gene function. However, affordable technology for proteome-scale antibody generation does not exist. To address this, we developed Proteome Epitope Tag Antibody Library (PETAL) and its array. PETAL consists of 62,208 monoclonal antibodies (mAbs) against 15,199 peptides from diverse proteomes. PETAL harbors binders for a great multitude of proteins in nature due to antibody multispecificity, an intrinsic antibody feature. Distinctive combinations of 10,000 to 20,000 mAbs were found to target specific proteomes by array screening. Phenotype-specific mAb-protein pairs were found for maize and zebrafish samples. Immunofluorescence and flow cytometry mAbs for membrane proteins and chromatin immunoprecipitation-sequencing mAbs for transcription factors were identified from respective proteome-binding PETAL mAbs. Differential screening of cell surface proteomes of tumor and normal tissues identified internalizing tumor antigens for antibody-drug conjugates. By finding high-affinity mAbs at a fraction of current time and cost, PETAL enables proteomescale antibody generation and target discovery.
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