文献类型： 外文期刊
作者： Chen, Mei-chun 1 ; Liu, Tian-tian 2 ; Wang, Jie-ping 1 ; Chen, Yan-ping 1 ; Chen, Qing-xi 2 ; Zhu, Yu-jing 1 ; Liu, Bo 1 ;
作者机构： 1.Fujian Acad Agr Sci, Agr Bioresources Res Inst, Fuzhou 350003, Peoples R China
2.Xiamen Univ, Coll Biol Sci & Engn, Xiamen 361005, Peoples R China
关键词： Lipopeptides; inhibitory activities; lipase; action mode
期刊名称：JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY （ 影响因子：5.051； 五年影响因子：4.598 ）
ISSN： 1475-6366
年卷期： 2020 年 35 卷 1 期
页码：
收录情况： SCI
摘要： Lipopeptides have been reported to exhibit anti-obesity effects. In this study, we obtained a Bacillus velezensis strain FJAT-52631 that could coproduce iturins, fengycins, and surfactins. Results showed that the FJAT-52631 crude lipopeptide, purified fengycin, iturin, and surfactin standards exhibited strong inhibition activities against lipase with dose-dependence manners (half maximal inhibitory concentration (IC50) = 0.011, 0.005, 0.056, and 0.005 mg/mL, respectively). Moreover, fengycin and surfactin had the comparable activities with orlistat, but iturin not. It was revealed that the inhibition mechanism and type of the lipopeptides were reversible and competitive. The quenching mechanism of lipase was static and only one binding site between lipase and lipopoeptide was inferred from the fluorescence analysis. The docking analysis displayed that fengycin and surfactin could directly interact with the active amino acid residues (Ser or Asp) of lipase, but not with iturin. Our work suggests that the B. velezensis lipopeptides would have great potential to act as lipase inhibitors.
- 相关文献
作者其他论文 更多>>
-
Lipopeptides from Bacillus velezensis induced apoptosis-like cell death in the pathogenic fungus Fusarium concentricum
作者：Chen, Meichun;Deng, Yingjie;Xiao, Rongfeng;Liu, Bo;Wang, Jieping;Deng, Yingjie;Wang, Xun;He, Jin;Deng, Yingjie;Wang, Xun;He, Jin;Zheng, Meixia
关键词：crop protection; antifungal peptides; lipopeptides; pathogenic fungus; Fusarium concentricum; cell apoptosis
-
Accurate Prediction of Dissolved Oxygen in Perch Aquaculture Water by DE-GWO-SVR Hybrid Optimization Model
作者：Bao, Xingsheng;Wu, Renye;Jiang, Yilun;Qu, Fangfang;Zhang, Lintong;Chen, Linjie;Zhang, Wenqing;Xie, Lihang;Liu, Xinze;Qu, Fangfang;Liu, Bo
关键词：dissolved oxygen; water quality prediction; differential evolution; gray wolf optimizer
-
Structural Characteristic, Strong Antioxidant, and Anti-Gastric Cancer Investigations on an Oleoresin from Ginger (Zingiber officinale var. roscoe)
作者：Chen, Meichun;Lin, Enquan;Xiao, Rongfeng;Liu, Bo;Wang, Jieping;Li, Zuliang
关键词：ginger oleoresin; volatile components; phenolic compounds; antioxidant; cell proliferation and apoptosis
-
Lipopeptide C17 Fengycin B Exhibits a Novel Antifungal Mechanism by Triggering Metacaspase-Dependent Apoptosis in Fusarium oxysporum
作者：Deng, Ying-jie;Chen, Yan-ping;Wang, Jie-ping;Xiao, Rong-feng;Liu, Bo;Chen, Mei-chun;Deng, Ying-jie;Wang, Xun;He, Jin;Deng, Ying-jie;Wang, Xun;He, Jin;Chen, Zheng
关键词：Bacillus; lipopeptide; fengycin; pathogenic fungus; Fusarium oxysporum; cellapoptosis; metacaspase-dependent
-
Temporal dynamics of microbial composition and antibiotic resistome in fermentation bed culture pig farms across various ages
作者：Pan, Zhizhen;Wang, Weiyi;Chen, Jingyu;Avellan-Llaguno, Ricardo David;Xu, Wenjuan;Duan, Yifang;Huang, Qiansheng;Chen, Zheng;Liu, Bo;Chen, Jingyu;Wang, Weiyi
关键词：Fermentation bed culture; Microbial community; Antibiotic resistome; Bacterial pathogens; Metagenome-assembled genome
-
Testing a biocontrol agent consortium for suppression of tomato bacterial wilt through rhizosphere microecological regulation
作者：Zheng, Xuefang;Wang, Jieping;Chen, Meichun;Chen, Yanping;Chen, Zheng;Liu, Bo;Wang, Mingkuang
关键词：Bacterial wilt; Biocontrol control agent consortium; Microecological regulation; Ralstonia solanacearum; Rhizosphere
-
Engineering of Bacillus thuringiensis Cry2Ab toxin for improved insecticidal activity
作者：Xu, Lian;Zheng, Mei-Xia;Zhu, Yu-Jing;Fu, Bai-Wen;Shi, Yan
关键词：Cry2Ab; Protein engineering; Oligomerization; Pore-forming activity; Insecticidal activity
