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Specific PCR-based Marker for Identification of Common Carp 'Hainan' in China

文献类型： 外文期刊

作者： Laghari, Muhammad Younis 1 ; Zhao, Zixia 1 ; Gong, Yiwen 4 ; Lashari, Punhal 1 ; Li, Shangqi 1 ; Xu, Jian 1 ; Khooharo 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Aquat Genom, CAFS Key Lab Aquat Genom, Minist Agr, Beijing 100141, Peoples R China

2.Chinese Acad Fishery Sci, Beijing Key Lab Fishery Biotechnol, Beijing 100141, Peoples R China

3.Univ Sindh, Dept Freshwater Biol & Fisheries, Jamshoro 76080, Pakistan

4.Shanghai Ocean Univ, Natl Demonstrain Ctr Expt Fisheries Sci Educ, Shanghai, Peoples R China

5.Karachi Univ, Ctr Excellence Marine Biol, Sindh, Pakistan

关键词： Common carp; Genetic diversity; Hainan population; PCR; Specific molecular marker

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）

ISSN： 0030-9923

年卷期： 2020 年 52 卷 3 期

页码：

收录情况： SCI

摘要： The common carp (Cyprinus carpio L.) is one of the most important aquaculture species in the world. In China, due to the geographical isolation of Hainan island from the mainland, the genetic diversity in Hainan island is different to that in the mainland. One of fifty sequences isolated from SINE element was polymorphic, including six haplotypes with an average sequence divergence of 11.03% (SE = 0.78%). Four haplotypes (MF177501-MF177504) were shared in these four population. However, two specific haplotypes (MF177499-MF177500) were only observed in Hainan population. To simplify procedure and improve effectiveness of identifying the Hainan population, one specific PCR-based marker acted as INDEL marker was developed and then 120 samples were used as templates for PCR validation. The specific marker will facilitate the assessment of genetic diversity and selective breeding programs for Hainan common carp.

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