Identification and complete genomic sequence of a novel sadwavirus discovered in pineapple (Ananas comosus)
文献类型： 外文期刊
作者： Larrea-Sarmiento, Adriana 1 ; Olmedo-Velarde, Alejandro 1 ; Green, James C. 1 ; Al Rwahnih, Maher 2 ; Wang, Xupe 1 ;
作者机构： 1.Univ Hawaii, Dept Plant & Environm Protect Sci, Honolulu, HI 96822 USA
2.Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
3.Chinese Acad Trop Agr Sci, South Subtrop Crop Res Inst, Zhanjiang, Peoples R China
4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou, Hainan, Peoples R China
5.Guangdong Acad Agr Sci, Inst Plant Protect, Key Lab New Tech Plant Protect Guangdong, Guangzhou, Peoples R China
6.ARS, USDA, Pacific Basin Agr Res Ctr, Hilo, HI USA
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2020 年 165 卷 5 期
页码：
收录情况： SCI
摘要： The complete genomic sequence of a putative novel member of the family Secoviridae was determined by high-throughput sequencing of a pineapple accession obtained from the National Plant Germplasm Repository in Hilo, Hawaii. The predicted genome of the putative virus was composed of two RNA molecules of 6,128 and 4,161 nucleotides in length, excluding the poly-A tails. Each genome segment contained one large open reading frame (ORF) that shares homology and phylogenetic identity with members of the family Secoviridae. The presence of this new virus in pineapple was confirmed using RT-PCR and Sanger sequencing from six samples collected in Oahu, Hawaii. The name "pineapple secovirus A" (PSVA) is proposed for this putative new sadwavirus.
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