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Engineering Herbicide-Tolerance Rice Expressing an Acetohydroxyacid Synthase with a Single Amino Acid Deletion

文献类型： 外文期刊

作者： Fang, Jun 1 ; Wan, Changzhao 1 ; Wang, Wei 2 ; Ma, Liuyin 3 ; Wang, Xinqi 1 ; Cheng, Can 1 ; Zhou, Jihua 1 ; Qiao, Yongji 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Crop Breeding & Cultivating Inst, 1000 Jingqi Rd, Shanghai 201403, Peoples R China

2.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, 99 Haike Rd, Shanghai 201210, Peoples R China

3.Fujian Agr & Forestry Univ, Basic Forestry & Prote Res Ctr, Haixia Inst Sci & Technol, Fuzhou 350002, Fujian, Peoples R China

关键词： AHAS activity; the W548 deletion; multi-herbicide tolerance; rice

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 4 期

页码：

收录情况： SCI

摘要： The acetohydroxyacid synthase (AHAS) is an essential enzyme involved in branched amino acids. Several herbicides wither weeds via inhibiting AHAS activity, and the AHAS mutants show tolerance to these herbicides. However, most AHAS mutations are residue substitutions but not residue deletion. Here, residue deletion was used to engineering the AHAS gene and herbicide-tolerant rice. Molecular docking analysis predicted that the W548 of the AHAS was a residue deletion to generate herbicide tolerance. The AHAS-Delta W548 protein was generated in vitro to remove the W548 residue. Interestingly, the deletion led to the tetramer dissociation of the AHAS, while this dissociation did not reduce the activity of the AHAS. Moreover, the W548 deletion contributed to multi-family herbicides tolerance. Specially, it conferred more tolerance to sulfometuron-methyl and bispyribac-sodium than the W548L substitution. Further analysis revealed that AHAS-Delta W548 had the best performance on the sulfometuron-methyl tolerance compared to the wild-type control. Over-expression of the AHAS-Delta W548 gene into rice led to the tolerance of multiple herbicides in the transgenic line. The T-DNA insertion and the herbicide treatment did not affect the agronomic traits and yields, while more branched-chain amino acids were detected in transgenic rice seeds. Residue deletion of W548 in the AHAS could be a useful strategy for engineering herbicide tolerant rice. The increase of branched-chain amino acids might improve the umami tastes of the rice.

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