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Variations in colchicine-induced autotetraploid plants of Lilium davidii var. unicolor

文献类型： 外文期刊

作者： Li, Shujie 1 ; Lin, Yuhong 3 ; Pei, Huaidi 3 ; Zhang, Jingjing 4 ; Zhang, Jinwen 1 ; Luo, Junjie 3 ;

作者机构： 1.Gansu Agr Univ, Coll Agron, Lanzhou 730070, Peoples R China

2.Gansu Prov Key Lab Aridland Crop Sci, Gansu Key Lab Crop Improvement & Germplasm Enhanc, Lanzhou 730070, Peoples R China

3.Gansu Acad Agr Sci, Inst Biotechnol, Lanzhou 730070, Peoples R China

4.Gansu Agr Univ, Coll Resources & Environm Sci, Lanzhou 730070, Peoples R China

关键词： Lanzhou lily (Lilium davidii var; unicolor); Colchicine; Tetraploid induction rate; Variation

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期：

页码：

收录情况： SCI

摘要： Colchicine-induced polyploid plants exhibit more variations not only in morphology and cytology but also in genomic DNA sequence. In this study, tetraploid Lanzhou lily (Lilium davidii var. unicolor) plants were induced by colchicine, and the factors that affected the tetraploid induction rate, including pre-culture duration and colchicine concentration, were examined. The results showed that both pre-culture duration and colchicine concentration exerted significant effects on the tetraploid induction rate. The highest tetraploid induction rate (33.33%) resulted from a 15-day-preculture treatment followed by soaking in 0.025% colchicine solution for 24 h. The ploidy levels of the regenerated plantlets were confirmed by flow cytometry analysis and chromosome number counting. The variations in morphological and cytological characteristics, as well as genomic DNA sequences of colchicine-induced tetraploids and diploids, were compared. The results indicated that tetraploids shared less leaf numbers, more leaf width, lower stomata density, and longer guard cell length. Moreover, inter-simple sequence repeat assay revealed notable genetic variations in the genomic DNA sequence between tetraploids and diploids, and the 75% polymorphism between them indicated that the variation in DNA sequence occurred during the chromosome doubling. Key message Colchicine-induced tetraploid Lanzhou lily plants exhibited variations not only in morphology (including leaf number and leaf width) and cytology (including stomata density and guard cell length) but also in genomic DNA sequence.

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