Research on the Callus Formation Conditions of the Codonopsis pilosula Induced by Nano-TiO2
文献类型： 外文期刊
作者： Zhao, Ying 1 ; Zhao, Ping 2 ; Luo, Junjie 1 ; Tian, Li 3 ; Tian, Zhenji 4 ;
作者机构： 1.Gansu Acad Agr Sci, Inst Biotechnol, Lanzhou 730070, Gansu, Peoples R China
2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China
3.Univ Manitoba, Fac Agr & Food Sci, Winnipeg, MB R3T 2N2, Canada
4.Lanzhou Univ Technol, Sch Sci, Lanzhou 730050, Gansu, Peoples R China
关键词： Codonopsis pilosula; Callus; Induction; Conditions; Nano-TiO2
期刊名称：JOURNAL OF BIOBASED MATERIALS AND BIOENERGY （ 影响因子：0.708； 五年影响因子：0.739 ）
ISSN： 1556-6560
年卷期： 2019 年 13 卷 2 期
页码：
收录情况： SCI
摘要： A method for induction of the Codonopsis pilosula callus and its subculture was established. Seeds were sterilized with different methods for inducing aseptic seedling. The different parts of plants and C. pilosula aseptic seedlings were used for the explants, and various concentrations of auxin NAA, cytokinin 6-BA and nano-TiO2 combination on MS medium were used for callus induction, then the calluses with better growth status were selected for secondary culture. The best disinfectant was mercuric chloride. Seeds whose germination rate was up to 65.2% were disinfected with 75% of ethanol for 40 s, then disinfected 20 min with 1.5% of NaClO, and then with 0.1% of HgCl2 solution disinfected for 3 min. Aseptic seedling hypocotyl as explants was the best for callus induction, adding 1 mg/L 6-BA and 1.5 mg/L of NAA and 0.02 mg/L of nano-TiO2 to MS medium, and induced rate was nearly 100%. Nano-TiO2 was effective in preventing bacterial contamination of MS medium. Calluses which were induced by cotyledon serving as explants appeared the most severe browning. Thereafter, the optimal hormone combination of C. pilosula subculture was 2 mg/L 6-BA, 1 mg/L NAA and 0.02 mg/L Nano-TiO2.
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