Effects of Different Hormone Concentrations on Callus Induction of Astragalus mongholicus Bunge
文献类型： 外文期刊
作者： Zhao, Ying 1 ; Zhao, Ping 2 ; Luo, Junjie 1 ; Tian, Li 3 ; Tian, Zhenji 4 ; Li, Jingwen 1 ;
作者机构： 1.Gansu Acad Agr Sci, China Inst Biotechnol, Lanzhou 730070, Peoples R China
2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China
3.Univ Manitoba, Fac Agr & Food Sci, Winnipeg, MB R3T 2N2, Canada
4.Lanzhou Univ Technol, Sch Sci, Lanzhou 730050, Peoples R China
关键词： Mongolian Astragalus; Callus; Induction; Hormone; Nano-TiO2
期刊名称：NANOSCIENCE AND NANOTECHNOLOGY LETTERS （ 影响因子：1.128； 五年影响因子：0.985 ）
ISSN： 1941-4900
年卷期： 2019 年 11 卷 12 期
页码：
收录情况： SCI
摘要： A method of callus induction and subculture of Astragalus mongholicus was established. Different methods were used to sterilize seeds to induce sterile seedlings. The different parts of plants and Astragalus aseptic seedlings were used for the explants, different concentrations of auxin NAA and Cytokinin BAP were used in MS medium to induce callus, and then the callus with better growth condition was selected for secondary culture. Results showed that the browning phenomenon in the secondary culture was inhibited and the best disinfectant was mercuric chloride. The germination rate of seeds treated with 75% ethanol for 40 s, 1.5% NaCl for 20 minutes, and 0.1% HgCl2 for 3 minutes can reach up to 66.2%. The hypocotyls of sterile seedlings as explants were the best way to induce callus. The induction rate was close to 100% when 0.5 mg/L BAP and 0.15 mg/L of NAA and 0.01 mg/L of nano-TiO2 were added to MS medium. Besides, Nano-TiO2 can effectively prevent bacterial contamination of MS medium. Cotyledons, induced by cotyledon as explants, showed the most severe browning. Therefore, the best hormone combination of Astragalus subculture was 1.0 mg/L BAP, 1.5 mg/L NAA and 0.01 mg/L nano-TiO2.
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