文献类型： 外文期刊
作者： Zhu, Xiang-yun 1 ; Luo, Yan-ping 2 ; Xu, Bin 1 ; Ma, Bao-shan 1 ; Wei, Kai-jin 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Hubei, Peoples R China
2.Wuhan Inst Design & Sci, Wuhan 430205, Hubei, Peoples R China
关键词： Procambarus Clarkii; Growth Trait Correlation; Gene Cloning; Single Nucleotide Polymorphism; Association Analysis
期刊名称：REVISTA CIENTIFICA-FACULTAD DE CIENCIAS VETERINARIAS （ 影响因子：0.444； 五年影响因子：0.473 ）
ISSN： 0798-2259
年卷期： 2020 年 30 卷 2 期
页码：
收录情况： SCI
摘要： Using molecular genetic markers to analyze the genetic basis of the growth characters of procambarus crustaceans and to find the markers or genes associated with the excellent growth characters is an important part of the study on the genetic breeding and breed improvement of procambarus crustaceans. The purpose of this paper is to find SNP markers related to growth traits for marker assisted selection based on EST sequence of cDNA library of procyanus clarkii. In this paper, the rapid amplification technology of cDNA terminal (RACE) was applied to the cathepsin L gene, trypsin gene and haidian amylase gene of procrayfish. Rt-pcr was used to detect the distribution of the genes in each tissue of procambarus clarkii and their expression characteristics in each stage of ovarian development or growth, so as to explore the expression regulation mechanism and function of these genes in procambarus clarkii. Through the screening of SNPS by resequencing in four wild populations, Sna Pshot technology was used to detect the typing of candidate SNP sites, and the correlation analysis of genotype results and growth traits was carried out, and the expression level was all less than 0.5. This study is helpful to accelerate the process of genetic improvement and breeding of new species.
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