文献类型： 外文期刊

作者： Zhang, Kai ¹ ; Cui, Xiaozhen ² ; Zhang, Bochi ² ; Song, Xianyi ² ; Liu, Qiang ¹ ; Yang, Shiyu ³ ;

作者机构： 1.Shanxi Agr Univ, Coll Anim Sci & Vet Med, Taigu 030801, Shanxi, Peoples R China

2.Shanxi Acad Agr Sci, Feed & Vet Med Res Inst, Taiyuan 030036, Shanxi, Peoples R China

3.UCL Inst Neurol, Dept Clin Neurosci, Rowland Hill St, London NW3 2PF, England

关键词： Neural crest-derived stem cells; Adipose; Bovine; Multipotent; Differentiation

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN： 0006-291X

年卷期： 2020 年 522 卷 4 期

页码：

收录情况： SCI

摘要： Neural crest stem cells (NCSCs) often referred to as the fourth germ layer, comprise a migratory, stem and progenitor cell population and are synonymous with vertebrate evolution and development. The cells follow specific paths to migrate to different locations of the body where they generate a diverse array of cell types and tissues. There are NCSCs which are maintained in an undifferentiated state throughout the life in the animal tissues. Based on some cells migratory property, we successfully developed a separation strategy to isolate and identify a population of adipose-derived stem cells with neural crest stem cell features in adult bovine adipose tissues within minimally-invasive surgical procedures. The cells have a high degree of multi-potency and self-renewal capabilities, can be cultured and maintained in feeder-free adhesion conditions as monolayer cells, and also be able to grow in the suspension condition in the form of neurosphere. For the purpose of simple description, we name this type cell as bovine adipose-derived neural crest stem cell (baNCSC). Taken together our study describes a readily accessible source of multipotent baNCSC for autologous tissue engineer and cell-based therapeutic researches. (C) 2019 Elsevier Inc. All rights reserved.