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Functional characterization of maize heat shock transcription factor gene ZmHsf01 in thermotolerance

文献类型： 外文期刊

作者： Zhang, Huaning 1 ; Li, Guoliang 1 ; Hui, Dong 1 ; Zhang, Yuanyuan 2 ; Zhang, Yujie 3 ; Shao, Hongbo 4 ; Zhao, Lina 2 ; Ya 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Plant Genet Engn Ctr Hebei Prov, Shijiazhuang, Hebei, Peoples R China

2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang, Hebei, Peoples R China

3.Hebei North Univ, Coll Agr & Forestry Sci & Technol, Zhangjiakou, Peoples R China

4.JAAS, Salt Soil Agr Ctr, Inst Agr Resources & Environm, Key Lab Agr Environm Lower Reaches Yangtze River, Nanjing, Peoples R China

5.Yancheng Teachers Univ, Jiangsu Synthet Innovat Ctr Coastal Bioagr, Jiangsu Key Lab Bioresources Saline Soils, Yancheng, Jiangsu, Peoples R China

6.Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao, Peoples R China

关键词： Thermotolerance; Heat shock transcription factors; Maize; ABA; Subcellular localization; H2O2; Adversity stresses; Signal transduction pathway; Functional analysis; ZmHsf01

期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）

ISSN： 2167-8359

年卷期： 2020 年 8 卷

页码：

收录情况： SCI

摘要： Background. Heat waves can critically influence maize crop yields. Plant heat shock transcription factors (HSFs) play a key regulating role in the heat shock (HS) signal transduction pathway. Method. In this study, a homologous cloning method was used to clone HSF gene ZmHsf01 (accession number: MK888854) from young maize leaves. The transcript levels of ZmHsf01 were detected using qRT-PCR in different tissues and treated by HS, abscisic acid (ABA), hydrogen peroxide (H2O2), respectively, and the functions of gene ZmHsf01 were studied in transgenic yeast and Arabidopsis. Result. ZmHsf01 had a coding sequence (CDS) of 1176 bp and encoded a protein consisting of 391 amino acids. The homologous analysis results showed that ZmHsf01 and SbHsfA2d had the highest protein sequence identities. Subcellular localization experiments confirmed that ZmHsf01 was localized in the nucleus. ZmHsf01 was expressed in many maize tissues. It was up-regulated by HS, and up-regulated in roots and down-regulated in leaves under ABA and H2O2 treatments. ZmHsf01-overexpressing yeast cells showed increased thermotolerance. In Arabidopsis seedlings, ZmHsf01 compensated for the thermotolerance defects of mutant athsfa2, and ZmHsf01-overexpressing lines showed enhanced basal and acquired thermotolerance. When compared to wild type (WT) seedlings, ZmHsf01-overexpressing lines showed higher chlorophyll content and survival rates after HS. Heat shock protein (HSP) gene expression levels were more up-regulated in ZmHsf01-overexpressing Arabidopsis seedlings than WT seedlings. These results suggest that ZmHsf01 plays a vital role in response to HS in plant.

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