Expression Profiles of dmrt1 in Schizothorax kozlovi, and Their Relation to CpG Methylation of Its Promoter and Temperature
文献类型： 外文期刊
作者： He, Yongfeng 1 ; Wu, Xingbing 1 ; Zhu, Yongjiu 1 ; Yang, Deguo 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr & Rural Affairs China, 8,1st Wudayuan Rd, Wuhan 430223, Hubei, Peoples R China
关键词： Schizothorax kozlovi; dmrt1; expression profile; methylation; temperature
期刊名称：ZOOLOGICAL SCIENCE （ 影响因子：0.931； 五年影响因子：1.036 ）
ISSN： 0289-0003
年卷期： 2020 年 37 卷 2 期
页码：
收录情况： SCI
摘要： To elucidate the role of dmrt1 in sex differentiation of a teleost fish Schizothorax kozlovi, the full-length sequences of its cDNA and promoter were cloned by rapid amplification of cDNA ends (RACE) and genome walking. The relative mRNA expression levels were determined by quantitative real-time PCR (RT-PCR). The 1095-bp dmrt1 cDNA was predicted to encode a protein of 264 amino acids. It was expressed only in the gonads, and the expression was 17-times higher in the testis than in the ovary. The 1215-bp promoter sequence of dmrt1 was cloned and analyzed to detect sex-related differences in its methylation levels. A significant negative relationship between the dmrt1 expression and CpG methylation of its promoter were found in the testes and ovaries of S. kozlovi. Significant differences in dmrt1 expression levels were also found between the larval and juvenile stages. No significant differences in expression were found during the entire larval stage, and in the individuals among three different temperature groups (10 degrees C, 14 degrees C, and 18 degrees C). Considering that the sex of sampled larval fish cannot be distinguished, correlations between dmrt1 expression and effects of temperature on sex differentiation in S. kozlovi need further study.
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