Oral vaccination using Artemia coated with recombinant Saccharomyces cerevisiae expressing cyprinid herpesvirus-3 envelope antigen induces protective immunity in common carp (Cyprinus carpio var. Jian) larvae
文献类型： 外文期刊
作者： Ma, Yanping 1 ; Liu, Zhenxing 1 ; Hao, Le 1 ; Wu, Jing 2 ; Qin, Baotian 2 ; Liang, Zhiling 1 ; Ma, Jiangyao 1 ; Ke, Hao 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Key Lab Livestock Dis Prevent Guangdong Prov,Mini, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou 510640, Peoples R China
2.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China
3.Guangdong Ocean Univ, Zhanjiang 524088, Peoples R China
4.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510225, Peoples R China
关键词： Cyprinid herpesvirus 3; Saccharomyces cerevisiae surface display; pORF65 subunit vaccine; Artemia; Oral vaccination
期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）
ISSN： 0034-5288
年卷期： 2020 年 130 卷
页码：
收录情况： SCI
摘要： Cyprinid herpesvirus 3 (CyHV-3) is the etiological agent of koi herpersvirus disease (KHVD), which causes serious economic losses in global common carp and ornamental koi carp production of larvae as well as adult type fish. To control KHVD, vaccines against CyHV-3 utilizing different immunization routes have been developed, among them, oral vaccination is the most desirable method to prevent fish diseases occurring at the early larval stage. Here, we developed an oral subunit vaccine through the Saccharomyces cerevisiae cell surface display of CyHV-3 envelope protein pORF65, then, the recombinant yeast fed to Artemia which served as bio-encapsulation vector by subsequently feeding the common carp (Cyprinus carpio var. Jian) larvae. The fluorescent observation showed that the Artemia and S. cerevisiae could deliver intact antigen to the hindgut of carp larvae suggesting the possibility of the vector for oral immunization. On this basis, after three immunizations at a week interval, the oral vaccine induced high level of specific anti-pORF65 antibody. Meanwhile, a significant difference of immune-related genes expression occurred including cxca, IL-1 beta, IFN-alpha 1, lysozyme, IgM and CD8 alpha between vaccined group and blank control group. In addition, 30% of relative percent survival of carp larvae after immunization was obtained post the animal infection assay, offered an certain immune protection. Our results indicated that the oral pORF65 subunit vaccine bioencapsulated in Artemia induced the activation of immune response and high level of antibodies, which could be served as an oral vaccine candidate for the prevention of CyHV-3 infection.
- 相关文献
作者其他论文 更多>>
-
Characterization of a membrane Fcγ receptor in largemouth bass (Micropterus saloumoides) and its response to bacterial challenge
作者：Wang, Jingya;Wu, Jing;Li, Yugu;Wang, Jingya;Wu, Jing;Ma, Yanping;Hao, Le;Liu, Zhenxing;Wang, Jingya;Wu, Jing;Ma, Yanping;Hao, Le;Liu, Zhenxing;Huang, Wen
关键词：Membrane; Fc gamma R; Largemouth bass; Nocardia seriolae; Immune response
-
Long- and short-term dietary β-glucan improves intestinal health and disease resistance in pearl gentian grouper (Epinephelus lanceolatus♂ x Epinephelus fuscoguttatus♀)
作者：Wang, Fan;Wang, Zhiwen;Lu, Yishan;Wang, Fan;Wang, Fan;Wang, Zhiwen;Cao, Junming;Lu, Yishan;Cao, Junming
关键词：beta-Glucan; Administration period; Intestinal health; Fish
-
Response signatures of intestinal microbiota and gene transcription of the pearl gentian grouper to Vibrio harveyi infection
作者：Wang, Fan;Wang, Zhiwen;Lu, Yishan;Wang, Fan;Xu, Jia;Wang, Fan;Wang, Zhiwen;Lu, Yishan;Cao, Junming;Cao, Junming;Lu, Yishan
关键词：Transcriptome; Gut microbiotas; Vibrio harveyi; Grouper
-
Probiotic breakthrough: Clostridium butyricum improved the intestinal SCFAs content, digestive enzymes and antioxidant activities, gut morphology and microbiota composition of largemouth bass
作者：Chen, Xiaoying;Li, Peijia;Li, Min;Chen, Bing;Peng, Kai;Huang, Wen;Cao, Junming;Zhao, Hongxia;Li, Peijia
关键词：Clostridium butyricum; Largemouth bass; Growth performance; Intestinal health; Intestinal flora
-
Selenium Protects Yellow Catfish (Tachysurus fulvidraco) from Low-Temperature Damage via the Perspective Analysis of Metabolomics and Intestinal Microbes
作者：Hu, Junru;Wang, Guoxia;Zhao, Hongxia;Lu, Huijie;Peng, Kai;Huang, Wen;Sun, Yuping;Wang, Lei;Peng, Kai;Huang, Wen;Liu, Ding;Liu, Zhenxing
关键词：selenium; Tachysurus fulvidraco; low-temperature stress; metabolomics; intestinal microbes
-
The protective role of condensed tannins on growth inhibition and enterohepatic injury induced by histamine in Chinese seabass (Lateolabrax maculatus)
作者：Qiu, Jianqiang;Chen, Bing;Zhao, Hongxia;Huang, Wen;Cao, Junming;Wang, Guoxia;Peng, Kai;Qiu, Jianqiang;Loh, Jiun-Yan;Loh, Jiun-Yan
关键词：Condensed tannins; Detoxication; Histamine; Lateolabrax maculatus; Toxicity
-
Effects of sodium butyrate on growth performance, antioxidant status, inflammatory response and resistance to hypoxic stress in juvenile largemouth bass (Micropterus salmoides)
作者：Hou, Dongqiang;Li, Min;Li, Peijia;Chen, Bing;Huang, Wen;Cao, Junming;Zhao, Hongxia;Hou, Dongqiang;Li, Min;Li, Peijia;Guo, Hui
关键词：sodium butyrate; largemouth bass; antioxidant system; inflammatory response; hypoxic stress
