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Complete genomic sequence of Noni mosaic virus (NoMV) associated with a mosaic disease in Morinda citrifolia L.

文献类型： 外文期刊

作者： Yu, Nai-Tong 1 ; Cai, Zhi-Ying 2 ; Xiong, Zhongguo 3 ; Yang, Yan 2 ; Liu, Zhi-Xin 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Minist Agr & Rural Affairs, Haikou 571101, Hainan, Peoples R China

2.Yunnan Inst Trop Crops, Jinhong 666100, Peoples R China

3.Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA

4.Univ Arizona, Inst BIO5, Tucson, AZ 85721 USA

关键词： Noni mosaic virus; Potyvirus; Complete genome; Morinda citrifolia L; Illumina transcriptomic sequencing

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN： 0929-1873

年卷期： 2020 年 156 卷 4 期

页码：

收录情况： SCI

摘要： An outbreak of a virus-like disease has caused severe damage to noni plants (Morinda citrifolia L.) in Xishuangbanna area of Yunnan province in southwest China since 2015. The diseased plants displayed typical mosaic symptom with light and dark green patches on leaves. Flexuous filamentous virus particles of about 800 nm in length were observed from the leaf saps by transmission electron microscope. Illuemina transcriptomic sequencing further revealed the presence of a potyvirus and its near complete genome was obtained from de novo assembly. The complete genome of 9659 nts was obtained by Sanger sequencing of eight amplicons generate by RT-PCR and 5 ' and 3' RACE. BLASTp analysis of the polyprotein sequence showed that the virus was most closely related to Tobacco vein banding mosaic virus (TVBMV), but these two viruses only shared 50.7% amino acid sequence similarity. Both phylogenetic analyses of the polyprotein and CP amino acid sequences indicated that this virus is a member of genus Potyvirus. However, the low sequence homology with all known potyviruses established this virus as a new species in the genus, tentatively named as Noni mosaic virus (NoMV). Our field surveys showed that 100% of the symptomatic samples and 28.57% of the asymptomatic samples were infected with this novel potyvirus. Aphids collected from diseased leaves were also detected carrying the virus and aphid transmission test confirmed it can transmit the NoMV. In summary, our data indicated that a novel species of potyvirus, NoMV, is prevalent in Yunnan, China and is associated with an emerging mosaic disease on M. citrifolia.

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