您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation

文献类型： 外文期刊

作者： Chen, Genghua 1 ; Chen, Jiahui 1 ; Qi, Lin 1 ; Yin, Yunqian 1 ; Lin, Zetong 1 ; Wen, Huaqiang 1 ; Zhang, Shuai 1 ; Xiao, Chuanyun 6 ; Bello, Semiu Folaniyi 1 ; Zhang, Xiquan 1 ; Nie, Qinghua 1 ; Luo, Wen 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China

2.Minist Agr, Lingnan Guangdong Lab Modern Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Guangzhou, Guangdong, Peoples R China

3.Minist Agr, State Key Lab Conservat & Utilizat Subtrop Agrobio, Key Lab Chicken Genet Breeding & Reprod, Guangzhou, Guangdong, Peoples R China

4.South China Agr Univ, State Key Lab Livestock & Poultry Breeding, Guangzhou, Peoples R China

5.South China Agr Univ, Lingnan Guangdong Lab Agr, Guangzhou, Peoples R China

6.Wageningen Univ, Human & Anim Physiol, Wageningen, Netherlands

7.South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

期刊名称：CELL PROLIFERATION （ 影响因子：8.5； 五年影响因子：7.8 ）

ISSN： 0960-7722

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Alternative splicing (AS) disruption has been linked to disorders of muscle development, as well as muscular atrophy. However, the precise changes in AS patterns that occur during myogenesis are not well understood. Here, we employed isoform long-reads RNA-seq (Iso-seq) and single-cell RNA-seq (scRNA-seq) to investigate the AS landscape during myogenesis. Our Iso-seq data identified 61,146 full-length isoforms representing 11,682 expressed genes, of which over 52% were novel. We identified 38,022 AS events, with most of these events altering coding sequences and exhibiting stage-specific splicing patterns. We identified AS dynamics in different types of muscle cells through scRNA-seq analysis, revealing genes essential for the contractile muscle system and cytoskeleton that undergo differential splicing across cell types. Gene-splicing analysis demonstrated that AS acts as a regulator, independent of changes in overall gene expression. Two isoforms of splicing factor TRA2B play distinct roles in myogenic differentiation by triggering AS of TGFBR2 to regulate canonical TGF-beta signalling cascades differently. Our study provides a valuable transcriptome resource for myogenesis and reveals the complexity of AS and its regulation during myogenesis.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台