您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Transcriptomic insights into the potential impacts of flavonoids and nodule-specific cysteine-rich peptides on nitrogen fixation in Vicia villosa and Vicia sativa

文献类型： 外文期刊

作者： Ren, Jian 1 ; Cui, Zhengguo 3 ; Wang, Yueqiang 3 ; Ning, Qiushi 4 ; Gao, Yingzhi 1 ;

作者机构： 1.Northeast Normal Univ, Inst Grassland Sci, Jilin Songnen Grassland Ecosyst Natl Observat & Re, Key Lab Vegetat Ecol,Minist Educ,State Environm Pr, Changchun 130024, Peoples R China

2.Xinjiang Agr Univ, Coll Grassland Sci, Key Lab Grassland Resources & Ecol Western Arid De, Minist Educ, Urumqi 830052, Peoples R China

3.Jilin Acad Agr Sci, Natl Engn Res Ctr Soybean, Soybean Res Inst, Changchun 130033, Peoples R China

4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China

关键词： Legumes; Forages; Nitrogen fixation; Nodulation; Root nodules; Comparative transcriptome

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.1； 五年影响因子：6.2 ）

ISSN： 0981-9428

年卷期： 2024 年 214 卷

页码：

收录情况： SCI

摘要： Vicia villosa (VV) and Vicia sativa (VS) are legume forages highly valued for their excellent nitrogen fixation. However, no research has addressed the mechanisms underlying their differences in nitrogen fixation. This study employed physiological, cytological, and comparative transcriptomic approaches to elucidate the disparities in nitrogen fixation between them. Our results showed that the total amount of nitrogen fixed was 60.45% greater in VV than in VS, and the comprehensive nitrogen response performance was 94.19% greater, while the nitrogen fixation efficiency was the same. The infection zone and differentiated bacteroid proportion in mature VV root nodules were 33.76% and 19.35% greater, respectively, than those in VS. The size of the VV genome was 15.16% larger than that of the VS genome, consistent with its greater biomass. A significant enrichment of the flavonoid biosynthetic pathway was found only for VV-specific genes, among which chalcone-flavonone isomerase, caffeoyl-CoA-O-methyltransferase and stilbene synthase were extremely highly expressed. The VV-specific genes also exhibited significant enrichment in symbiotic nodulation; genes related to nodule-specific cysteine-rich peptides (NCRs) comprised 61.11% of the highly expressed genes. qRT-PCR demonstrated that greater enrichment and expression of the dominant NCR (Unigene0004451) were associated with greater nodule bacteroid differentiation and greater nitrogen fixation in VV. Our findings suggest that the greater total nitrogen fixation of VV was attributed to its larger biomass, leading to a greater nitrogen demand and enhanced fixation physiology. This process is likely achieved by the synergistic effects of high bacteroid differentiation along with high expression of flavonoid and NCR genes.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台