文献类型： 外文期刊

作者： Dong, Xuan ¹ ; Li, Cixiu ⁵ ; Wang, Yiting ¹ ; Hu, Tao ⁵ ; Zhang, Fan ¹ ; Meng, Fanzeng ¹ ; Gao, Meirong ⁸ ; Han, Xuekai ⁸ ; Wang, Guohao ¹ ; Qin, Jiahao ¹ ; Nauwynck, Hans ⁹ ; Holmes, Edward C. ¹⁰ ; Sorgeloos, Patrick ⁹ ; Sui, Liying ⁸ ; Huang, Jie ¹ ; Shi, Weifeng ⁶ ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Peoples R China

2.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab Maricultural Organism Dis Control, Qingdao 266071, Peoples R China

4.Qingdao Key Lab Mariculture Epidemiol & Biosecur, Qingdao 266071, Peoples R China

5.Shandong First Med Univ, Shandong Prov Hosp, Jinan 250021, Peoples R China

6.Shandong First Med Univ & Shandong Acad Med Sci, Key Lab Emerging Infect Dis Univ Shandong, Tai An 271000, Peoples R China

7.Dalian Ocean Univ, Dalian 116023, Peoples R China

8.Tianjin Univ Sci & Technol, Asian Reg Artemia Reference Ctr, Tianjin 300457, Peoples R China

9.Univ Ghent, B-9000 Ghent, Belgium

10.Univ Sydney, Sydney Inst Infect Dis, Sch Med Sci, Sydney 2006, Australia

11.Network Aquaculture Ctr Asia Pacific, Bangkok 10900, Thailand

12.Shandong First Med Univ & Shandong Acad Med Sci, Sch Publ Hlth, Jinan 250117, Peoples R China

关键词： brine shrimp; Artemia; virus diversity; novel virus discovery; global connectedness; hypersaline ecosystem

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：9.1； 五年影响因子：7.1 ）

ISSN： 1674-7305

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Brine shrimp (Artemia) has existed on Earth for 400 million years and has major ecological importance in hypersaline ecosystems. As a crucial live food in aquaculture, brine shrimp cysts have become one of the most important aquatic products traded worldwide. However, our understanding of the biodiversity, prevalence and global connectedness of viruses in brine shrimp is still very limited. A total of 143 batches of brine shrimp (belonging to seven species) cysts were collected from six continents including 21 countries and more than 100 geographic locations worldwide during 1977-2019. In total, 55 novel RNA viruses were identified, which could be assigned to 18 different viral families and related clades. Eleven viruses were dsRNA viruses, 16 were +ssRNA viruses, and 28 were-ssRNA viruses. Phylogenetic analyses of the RNA-directed RNA polymerase (RdRp) showed that brine shrimp viruses were often grouped with viruses isolated from other invertebrates and fungi. Remarkably, most brine shrimp viruses were related to those from different hosts that might feed on brine shrimp or share the same ecological niche. A notable case was the novel brine shrimp noda-like virus 3, which shared 79.25% (RdRp) and 63.88% (capsid proteins) amino acid identity with covert mortality nodavirus (CMNV) that may cause losses in aquaculture. In addition, both virome composition and phylogenetic analyses revealed global connectedness in certain brine shrimp viruses, particularly among Asia and Northern America. This highlights the incredible species diversity of viruses in these ancient species and provides essential data for the prevalence of RNA viruses in the global aquaculture industry. More broadly, these findings provide novel insights into the previously unrecognized RNA virosphere in hypersaline ecosystems worldwide and demonstrate that human activity might have driven the global connectedness of brine shrimp viruses.