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Rice black-streaked dwarf virus P10 induces membranous structures at the ER and elicits the unfolded protein response in Nicotiana benthamiana

文献类型： 外文期刊

作者： Sun, Zongtao 1 ; Yang, Di; Xie, Li 1 ; Sun, Liying 1 ; Zhang, Shanglin; Zhu, Qisong; Li, Junmin 1 ; Wang, Xu 2 ; Chen, Jianping;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Zhejiang Prov Key Lab Plant Virol,Minist Agr, Key Lab Biotechnol Plant Protect,Inst Virol & Bio, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Zhejiang Prov Key Lab Plant Virol,Minist Agr, Key Lab Biotechnol Plant Protect,Inst Virol & Bio

关键词： Rice black-streaked dwarf virus; Capsid protein P10; ER membrane modification; ER stress; Unfolded protein response

期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）

ISSN： 0042-6822

年卷期： 2013 年 447 卷 1-2 期

页码：

收录情况： SCI

摘要： Endoplasmic reticular (ER) membrane modifications play an important role in viral RNA replication and virion assembly but little is known about the involvement of ER-membrane remodeling in the infection cycle of fijiviruses in plant cells. The subcellular localization of Rice black-streaked dwarf virus outer capsid P10 was therefore examined using live-cell imaging. P10 fused to eGFP formed vesicular structures associated with ER membranes in Nicotiana benthamiana epidermal cells and in rice protoplasts. Subcellular fractionation experiments confirmed that P10 is an integral membrane protein. Three predicted transmembrane domains and two less-well-defined domains were each able to target eGFP to the ER. Disruption of the actin cytoskeleton with LatB, indicated that the maintenance of P10-induced membrane structures required the intact actin cytoskeleton. P10 induced the expression of ER stress marker genes, including ER stress-related chaperones and transcription factor, indicating that RBSDV P10 triggers ER stress and the unfolded protein response. (C) 2013 Elsevier Inc. All rights reserved.

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