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A fractionation procedure for identifying novel proteins induced by chill stress in Arabidopsis thaliana

文献类型： 外文期刊

作者： Sun, Liwen 1 ; Wang, Shichen 1 ; Xi, Jinghui 2 ; Yang, Shuo 1 ; Liu, Xiangguo 1 ; Chai, Xin 1 ; Xin, Hongbao 1 ; An, Baiy 1 ;

作者机构： 1.Jilin Univ, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130023, Peoples R China

2.Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China

关键词： Arabidopsis;Chill stress;Debris;Fractionation;Salt gradient fraction

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

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收录情况： SCI

摘要： Extraction of plant proteins using typical extraction buffers leaves insoluble debris that cannot be investigated by conventional 2-DE technologies. In this paper, we present a scalable, off-line procedure for extraction of Arabidopsis thaliana homogenates that can be used in combination with both in-gel digestion and mass spectrometry. Based on sequential NaCl gradients and strong detergent fractionation, this new strategy allowed detection of 11 novel proteins from Arabidopsis thaliana that were altered in response to chilling stress.

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