文献类型： 外文期刊
作者： Yan, Fei 1 ; Peng, Jiejun 1 ; Lu, Yuwen 1 ; Lin, Lin 1 ; Zheng, Hongying 1 ; Chen, Hairu 2 ; Chen, Jianping 1 ; Adams, Mi 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China
2.Yunnan Agr Univ, Plant Protect Coll, Kunming 650205, Peoples R China
3.Rothamsted Res, Dept Plant Pathol & Microbiol, Harpenden AL5 2JQ, Herts, England
期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）
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收录情况： SCI
摘要： Dicer-like proteins (DCLs) are involved in small RNA-mediated development and viral defense in plants. In model plants, at least four DCLs have been found and a number of studies have helped to understand their function. However, the function of the Dicer or DCLs in other plants is still unclear. Here, we report the full-length cDNA sequence of Brassica rapa ssp. chinensis DCL2 (BrDCL2) gene, which contains a 4,179 bp open reading frame (ORF) encoding a protein of 1,392 amino acids. At the 3' end of BrDCL2, clones with three different lengths of 3' untranslated region were found. An alternative splice variant of BrDCL2, BrDCL2sv, in which one intron was retained between exon9 and exon10, was also cloned. Because of a change in the coding sequence resulting in a premature terminal codon, BrDCL2sv was expected to translate a short peptide containing the whole DEXHc domain.
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