Proteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality
文献类型： 外文期刊
作者： Lv, Liangjie 1 ; Zhao, Aiju 1 ; Zhang, Yelun 1 ; Li, Hui 1 ; Chen, Xiyong 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Crop Genet & Breeding Lab Hebei, Shijiazhuang, Hebei, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.38； 五年影响因子：5.134 ）
ISSN： 2045-2322
年卷期： 2021 年 11 卷 1 期
页码：
收录情况： SCI
摘要： The regulation of wheat protein quality is a highly complex biological process involving multiple metabolic pathways. To reveal new insights into the regulatory pathways of wheat glutenin synthesis, we used the grain-filling period wheat grains of the near-isogenic lines NIL-723 and NIL-1010, which have large differences in quality, to perform a combined transcriptome and proteome analysis. Compared with NIL-1010, NIL-723 had 1287 transcripts and 355 proteins with significantly different abundances. Certain key significantly enriched pathway were identified, and wheat quality was associated with alanine, aspartate and glutamate metabolism, nitrogen metabolism and alpha-linolenic acid metabolism. Differentially expressed proteins (DEPs) or Differentially expressed genes (DEGs) in amino acid synthesis pathways were upregulated primarily in the glycine (Gly), methionine (Met), threonine (Thr), glutamic acid (Glu), proline (proC), cysteine (Cys), and arginine (Arg) synthesis and downregulated in the tryptophan (trpE), leucine (leuC), citrulline (argE), and ornithine (argE) synthesis. Furthermore, to elucidate changes in glutenin in the grain synthesis pathway, we plotted a regulatory pathway map and found that DEGs and DEPs in ribosomes (RPL5) and the ER (HSPA5, HYOU1, PDIA3, PDIA1, Sec24, and Sec31) may play key roles in regulating glutenin synthesis. The transcriptional validation of some of the differentially expressed proteins through real-time quantitative PCR analysis further validated the transcriptome and proteomic results.
- 相关文献
作者其他论文 更多>>
-
Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring
作者：Sun, Mengjing;Tong, Jingyang;Dong, Yan;Zhang, Xueyong;Hao, Chenyang;Xu, Xiaowan;Cao, Qiang;Rasheed, Awais;Cao, Shuanghe;Xia, Xianchun;He, Zhonghu;Hao, Yuanfeng;Sun, Mengjing;Ni, Zhongfu;Pu, Zongjun;Zheng, Jianmin;Zhang, Yelun;Rasheed, Awais;He, Zhonghu;Rasheed, Awais;Ali, Mohamed Badry
关键词：
-
Targeted metabolome and transcriptome analyses reveal changes in gibberellin and related cell wall-acting enzyme-encoding genes during stipe elongation in Flammulina filiformis
作者：Li, Hui;Yao, Sen;Xia, Weiwei;Ma, Xinbin;Shi, Lei;Ju, Huimin;Li, Ziyan;Zhong, Yingli;Tao, Yongxin;Shi, Lei;Ju, Huimin;Li, Ziyan;Zhong, Yingli;Xie, Baogui;Tao, Yongxin
关键词：Flammulina filiformis; gibberellin; stipe elongation; targeted metabolome; transcriptome
-
QTL Mapping Reveals Both All-Stage and Adult-Plant Resistance to Powdery Mildew in Chinese Elite Wheat Cultivars
作者：Xu, Xiaoting;Ni, Zhongqiu;Zou, Xinyu;Tong, Jingyang;Xu, Xiaowan;Dong, Yachao;Han, Bin;Li, Simin;Wang, Desen;Xia, Xianchun;He, Zhonghu;Hao, Yuanfeng;Zhang, Yelun;He, Zhonghu
关键词：Blumeria graminis f. sp. tritici; KASP marker; QTL analysis; SNP array; Triticum aestivum
-
Molecular characterization of stable QTL and putative candidate genes for grain zinc and iron concentrations in two related wheat populations
作者：Sun, Mengjing;Tong, Jingyang;Wang, Yue;Song, Jie;Rasheed, Awais;Li, Ming;Cao, Shuanghe;Xia, Xianchun;He, Zhonghu;Hao, Yuanfeng;Luo, Qiaoling;Zheng, Qi;Zhang, Yelun;Pu, Zongjun;Zheng, Jianmin;Liu, Lianzheng;Zhou, Anding;Rasheed, Awais;He, Zhonghu;Rasheed, Awais
关键词：
-
Molecular identification and validation of four stable QTL for adult-plant resistance to powdery mildew in Chinese wheat cultivar Bainong 64
作者：Xu, Xiaowan;Sun, Daojie;Cao, Qiang;Xu, Xiaowan;Ni, Zhongqiu;Zou, Xinyu;Xu, Xiaoting;Sun, Mengjing;Cao, Qiang;Tong, Jingyang;Ding, Fugong;Wang, Fengju;Dong, Yachao;Zhang, Luyan;Wang, Jiankang;Xia, Xianchun;He, Zhonghu;Hao, Yuanfeng;Zhang, Yelun;He, Zhonghu
关键词：
-
Combined analysis of metabolome and transcriptome of wheat kernels reveals constitutive defense mechanism against maize weevils
作者：Lv, Liangjie;Guo, Xiaorui;Zhao, Aiju;Liu, Yuping;Li, Hui;Chen, Xiyong
关键词：Triticum aestivum L; Sitophilus zeamais; metabolome; transcriptome; constitutive defense; flavonoids biosynthesis
-
Transcriptome and Proteome Analysis Revealed the Influence of High-Molecular-Weight Glutenin Subunits (HMW-GSs) Deficiency on Expression of Storage Substances and the Potential Regulatory Mechanism of HMW-GSs
作者：Zhao, Yun;Zhao, Jie;Hu, Mengyun;Sun, Lijing;Liu, Qian;Zhang, Yelun;Li, Qianying;Wang, Peinan;Li, Hui;Zhang, Yingjun;Ma, Wujun;Ma, Wujun;Gao, Huimin
关键词：wheat; HMW-GSs; seed storage substances; regulation mechanism; candidate genes
