文献类型： 外文期刊
作者： Wang, Z. H. 1 ; Wang, L. 1 ; Ye, Q. S. 1 ;
作者机构： 1.S China Normal Univ, Coll Life Sci, Guangdong Key Lab Biotechnol Plant Dev, Guangzhou 510631, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Floricultural Res Inst, Guangzhou 510640, Guangdong, Peoples R China
关键词： Dendrobium nobile Lindl.;In vitro flowering;Seedlings;Temperate orchid
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Dendrobium nobile Lindl. is a popular temperate Chinese orchid commonly marketed as a traditional medicinal plant. Seedlings of Dendrobium nobile Lindl. produced floral buds (33.3-34.8%) precociously on a defined basal medium (1/2 MS) containing paclobutrazol (PP) at 0.5mgLp# or thidiazuron (TDZ) at 0.1mgLp# within 4 months of culturing. The frequency of floral buds formation can be further increased to 95.6% by growing seedlings in a PN (PP 0.3mgLp# +NAA 0.5mgLp#)-containing medium followed by transfer onto 1/2 MS medium with PP and TDZ (PP +TDZ). However, flower developed was deformed under 25pC but it developed fully when grown in a lower temperature regime (23pC/18pC, light/dark) for 45 days. Under optimal condition, in vitro flowering was observed about 6 months after seed sowing.
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