文献类型： 外文期刊

作者： Luogen, C. ¹ ; Jianing, X. ¹ ; Jing, X. ¹ ; Ying, W. ¹ ; Zhongying, L. ² ;

作者机构： 1.Nanjing Normal Univ, Coll Life Sci, Nanjing, Peoples R China

2.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang, Peoples R China

关键词： Plutella xylostella;deltamethrin resistance;ubiquitin gene

期刊名称：JOURNAL OF APPLIED ENTOMOLOGY （ 影响因子：2.603； 五年影响因子：2.562 ）

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收录情况： SCI

摘要： Plutella xylostella (L.) has long been regarded as a very important universal pest of cruciferous crops. It is therefore important to understand its mechanisms of insecticide resistance. In our previous study, we obtained a differently expressed sequence with relatively high homology to the ubiquitin (UBI) gene as assessed via cDNA representational difference analysis (RDA). To further study this product, we cloned UBI open reading frame (ORF) sequences identified from both sexes of the deltamethrin-resistant strain (DRF and DRM) and the deltamethrin-sensitive strain (SF and SM) of P. xylostella. The deduced protein sequence of DRM was different from the other samples. The expression level was measured using real-time quantitative polymerase chain reaction (PCR) and Western blot. The results of both analyses revealed the expression levels, in order from highest to lowest, to be in SF, SM, DRF and DRM. This indicates that the long-term use of deltamethrin on P. xylostella results in decreased expression of the UBI gene.