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Identification, characterization and differential expression analysis of a pteridine synthesis related gene, Ccptps, in koi carp (Cyprinus carpio L.)

文献类型： 外文期刊

作者： Shi, Xi 1 ; Shan, Yashi 1 ; Ma, Xiao 1 ; Wu, Limin 1 ; Song, Hongmei 3 ; Wu, Qisheng 4 ; Li, Xuejun 1 ; Tian, Xue 1 ;

作者机构： 1.Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Peoples R China

2.Henan Normal Univ, Engn Technol Res Ctr Henan Prov Aquat Anim Cultiva, Xinxiang 453007, Peoples R China

3.Pearl River Fisheries Res Inst, Chinese Acad Fishery Sci, Key Lab Trop & Subtrop Fishery Resource Applicat &, Minist Agr, Guangzhou 510380, Peoples R China

4.Fisheries Res Inst Fujian, Xiamen, Peoples R China

关键词： Koi carp; Ccptps; Cloning; Pteridine synthesis; Color formation

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 1096-4959

年卷期： 2023 年 264 卷

页码：

收录情况： SCI

摘要： 6-pyruvoyl-tetrahydropterin synthase (PTPS) is the second key enzyme of the pteridine biosynthetic pathway and it plays vital roles in fish body color formation. In this study, Ccptps of koi carp (Cyprinus carpio L.) was cloned, identified and characterized. The full-length cDNA of Ccptps was 1140 bp and encodes for 139 amino acids. Multiple alignments revealed that the amino acids sequence of CcPTPS shared the highest identity to that of C. carpio, and Ccptps was clustered with cyprinid fishes in phylogenetic tree. Liver tissues of koi carp exhibited the highest expression of Ccptps, followed by muscle and skin tissues. During early developmental stages, the expression of Ccptps declined from 2 dph to 4 dph, and increased from 4 dph to 12 dph. The expressions of Ccptps in three color-related tissues (skin, scale and caudal fin) of whole red (WR) koi carp were significantly higher than that of whole while (WW) koi carp. Immunohistochemistry results of skin tissues showed that CcPTPS was mainly located in epidermis, stratum compactum of dermis and muscle layer, with the signal intensities in stratum compactum and muscle layer were stronger in WR koi carp compared to WW koi carp. Co-expressions of CcPTPS, CcSPR and CcXDH were detected in skin tissues of WW and WR koi carps, with CcPTPS exhibited stronger signal intensity compared to CcSPR and CcXDH. These findings imply that Ccptps is potentially involved in koi carp body color formation through the pteridine synthesis pathway.

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