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Assessment of the role of brine shrimp Artemia in white spot syndrome virus (WSSV) transmission

文献类型: 外文期刊

作者: Zhang, Jia-Song 1 ; Dong, Shuang-Lin 1 ; Dong, Yun-Wei 1 ; Tian, Xiang-Li 1 ; Cao, Yi-Cheng 2 ; Li, Zuo-Jia 2 ; Yan, D 1 ;

作者机构: 1.Ocean Univ China, Key Lab Mariculture, Minist Educ, Coll Fisheries, Qingdao 266003, Peoples R China

2.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

3.Ludong Univ, Dept Life Sci, Yantai 264025, Peoples R China

关键词: adhesion;aquatic organisms;brine;DNA;prawns;shellfish;transmission;vectors;viruses;Artemia;donkeys;Penaeus;Penaeus vannamei

期刊名称:VETERINARY RESEARCH COMMUNICATIONS ( 影响因子:2.459; 五年影响因子:2.352 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Challenge tests with Artemia four different development stages (nauplii, metanauplii, pseudoadults and adults) to white spot syndrome virus was carried out by immersion challenge and virus-phytoplankton adhesion route in order to asses the possibility of Artemia acting as a vector of WSSV to penaeid shrimp Litopenaeus vannamei postlarvae. The WSSV succeeded in infecting four stages Artemia, and nested-PCR detection for WSSV revealed positive results to virus-phytoplankton adhesion route. No mass mortalities were observed in penaeid shrimp postlarvae fed with WSSV-positive Artemia which exposed to WSSV by virus-phytoplankton adhesion route, whereas WSSV DNA detected in penaeid shrimp postlarvae by nested-PCR. By contrary, no WSSV-positive was detected in any animal fed with WSSV-negative Artemia. These results indicated that Artemia could serve as a vector in WSSV transmission.

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