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Characterization and partial sequence of a new furovirus of wheat in China

文献类型： 外文期刊

作者： Ye, R 1 ; Zheng, T 2 ; Chen, J 2 ; Diao, A 2 ; Adams, MJ 2 ; Yu, S 2 ; Antoniw, JF 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Virol Lab, Hangzhou 310021, Peoples R China

2.Zhejiang Acad Agr Sci, Virol Lab, Hangzhou 310021, Peoples R China; Fudan Univ, Dept Microbiol, Shanghai 200433, Peoples R China; AFRC, Inst Arable Crops Res, Crop & Dis Management Dept, Harpenden AL5 2JQ, Herts, England

关键词： Chinese wheat mosaic virus;Nucleotide sequence;Sbwmv;Serology;Mosaic virus;Proteins

期刊名称：PLANT PATHOLOGY （ 影响因子：2.59； 五年影响因子：2.924 ）

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收录情况： SCI

摘要： A soil-borne wheat virus causing severe mosaic and stunting symptoms on wheat in China has been characterized. It had been considered to be soil-borne wheat mosaic virus (SBWMV) because of its rod-shaped virions and similarities to epidemiology and host range. In this study, the virions purified from infected wheat tissue were approximately 20 nm in diameter and of two lengths (140-160 nm and 280-300 nm), with a coat protein of 19 kDa and two RNA components of approximately 7 and 3.5 kb. A rabbit antiserum was produced against the virus and a serological relationship to SBWMV from the USA (Oklahoma) was demonstrated. However, the coat protein was not recognized by most monoclonal antibodies against Oklahoma SBWMV in either ELISA, ISEM or Western blot analysis, indicating epitope differences. In RT-PCR experiments the viral nucleotide sequences were significantly different from those of SBWMV, and this was confirmed by partial sequencing of the cloned PCR fragments generated from RNA1 (c. 1100 nt) and RNA2 (c. 1400 nt), which showed homologies of about 79 and 63%, respectively, to corresponding regions of SBWMV. Because of these significant differences in serology and nucleotide sequence it is suggested that it is a new furovirus for which the name Chinese wheat mosaic virus (CWMV) is proposed.

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