Phenylpropanoid metabolism enzyme activities and gene expression in postharvest melons inoculated with Alternaria alternata
文献类型: 外文期刊
作者: Bai, Yujia 1 ; Feng, Zuoshan 1 ; Paerhati, Maerhaba 4 ; Wang, Jin 2 ;
作者机构: 1.Xinjiang Agr Univ, Crop Sci Xinjiang Agr Univ Postdoctoral Programme, Urumqi 830052, Xinjiang, Peoples R China
2.Xinjiang Agr Univ, Coll Food Sci & Pharm, Urumqi 830052, Xinjiang, Peoples R China
3.Xinjiang Agr Univ, Xinjiang Key Lab Postharvest Sci & Technol Fruits, Urumqi 830052, Xinjiang, Peoples R China
4.Xinjiang Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Urumqi 830091, Xinjiang, Peoples R China
关键词: Melon; Alternaria alternata; Disease resistance; Phenylpropanoid metabolism
期刊名称:APPLIED BIOLOGICAL CHEMISTRY ( 影响因子:3.206; 五年影响因子:2.398 )
ISSN: 2468-0834
年卷期: 2021 年 64 卷 1 期
页码:
收录情况: SCI
摘要: This study explored the mechanism of melon resistance to Alternaria alternata (A. alternata) infection in Jiashi and 86-1 melons. Melons were inoculated with A.alternata and the change in lesion diameter was measured. The changes in cinnamic acid-4-hydroxylase (C4H), phenylalanine ammonia lyase (PAL), and 4-coumaric acid coenzyme A ligase (4CL) activity and gene expression were studied in the pericarp tissues of Jiashi and 86-1 melons. The lesion diameter was smaller in Jiashi melon than in 86-1 melon, and the pericarp lesions were smaller than pulp lesions, indicating that Jiashi melon can resist A. alternata infection better than 86-1 melon. After inoculation with A. alternata, the C4H, PAL, and 4CL activities of Jiashi and 86-1 melons peaked in the middle and late storage period, and the peak was higher in Jiashi melons. The gene expression changes were consistent with the enzyme activity. The C4H, PAL, and 4CL gene expression was significantly higher in Jiashi melon pericarp than in 86-1 melon, and the C4H, PAL, and 4CL activities in Jiashi melon were positively correlated with their gene expression, confirming the role of phenylpropanoid metabolism enzymes in resistance to A. alternata.
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