您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Evaluation of reference genes for quantitative real-time PCR normalization in the Neopyropia (Pyropia) oomycete pathogen Pythium porphyrae

文献类型： 外文期刊

作者： Yang, Huichao 1 ; Yan, Yongwei 2 ; Weng, Peiwen 2 ; Sun, Congcong 2 ; Yu, Jiaxing 2 ; Tang, Lei 3 ; Li, Jie 2 ; Mo, Zhaolan 1 ;

作者机构： 1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc,Minist, Pilot Natl Lab Marine Sci & Technol Qingdao,Key L, Qingdao 266071, Shandong, Peoples R China

3.Ocean Univ China, Coll Marine Life Sci, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao 266003, Shandong, Peoples R China

4.Ocean Univ China, Sanya Oceanog Inst, Key Lab Trop Aquat Germplasm Hainan Prov, Sanya 572000, Peoples R China

关键词： Pythium porphyrae; Reference genes; Neopyropia (Pyropia) yezonesis; qRT-PCR; Normalized algorithms

期刊名称：JOURNAL OF APPLIED PHYCOLOGY （ 影响因子：3.404； 五年影响因子：3.834 ）

ISSN： 0921-8971

年卷期：

页码：

收录情况： SCI

摘要： Red rot disease, caused by the oomycete agent Pythium porphyrae, severely impacts the production of the alga Neopyropia (Pyropia). However, little is known about the pathogenetic mechanisms involved because of a lack of a genetic transformation system for this pathogen. Understanding the gene expression patterns of pathogens in response to different conditions is vital; however, this requires suitable reference genes for quantitative real-time polymerase chain reaction data normalization. In this study, 11 reference genes of P. porphyrae were evaluated at different developmental stages (vegetative growth, sporangia formation, zoospore formation, and zoospore release), temperatures (4, 15, 25, and 37 degrees C), salinities (0, 20, and 35), pHs (4, 7.5, and 10) and infection stages [5, 7, 9 days post infection (dpi)]. Various mathematical algorithms (Delta Delta-C-T comparison method, BestKeeper, NormFinder, and geNorm) were used to evaluate the stability of the reference gene expression. Of the 11 genes, those encoding gamma-Tubulin and alpha-Tubulin2 showed the best stability across the different developmental stages, Actin2 and 18S under different temperature conditions, GAPDH and EF1 alpha under different salinity conditions, GAPDH and EIF2 alpha under different pH conditions, and GAPDH and alpha-tubulin2 across different infection stages. Under all the tested conditions, GAPDH showed the best stability, indicating a reliable candidate as a universal reference gene for qRT-PCR analysis. To our knowledge, this is the first study to evaluate reference genes in P. porphyrae, with the aim of finding suitable candidate genes for the standardization of gene expression studies in this algal oomycete pathogen, and potentially other related pathogens.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台