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Increasingly amplified stimulation mediated by TaNAC69-B is crucial for the leaf senescence in wheat

文献类型： 外文期刊

作者： Li, Jingkun 1 ; Qiao, Hualiang 1 ; Yin, Pengcheng 1 ; Liu, Meng 1 ; Yang, Yifan 1 ; Li, Ke 1 ; Yang, Le 1 ; Yang, Chaosha 1 ; Zhao, Lifeng 1 ; Zhou, Shuo 2 ; Liu, Yongwei 1 ; Zhou, Chunjiang 1 ; Wang, Geng 1 ;

作者机构： 1.Hebei Normal Univ, Coll Life Sci, Hebei Collaborat Innovat Ctr Cell Signaling & Envi, Minist Educ Key Lab Mol & Cell Biol,Hebei Key Lab, Shijiazhuang 050024, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Biotechnol & Food Sci, Shijiazhuang 050051, Peoples R China

关键词： NAC transcription factor; leaf senescence; Triticum aestivum (wheat); ABA biosynthesis; transcriptional regulation

期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）

ISSN： 0960-7412

年卷期： 2023 年 114 卷 3 期

页码：

收录情况： SCI

摘要： Leaf senescence involves massive multidimensional alterations, such as nutrient redistribution, and is closely related to crop yield and quality. No apical meristem, Arabidopsis transcription activation factor, and Cup-shaped cotyledon (NAC)-type transcription factors integrate various signals and modulate an enormous number of target genes to ensure the appropriate progression of leaf senescence. However, few leaf senescence-related NACs have been functionally characterized in wheat. Based on our previous RNA-sequencing (RNA-seq) data, we focused on a NAC family member, TaNAC69-B, which is increasingly expressed during leaf senescence in wheat. Overexpression of TaNAC69-B led to precocious leaf senescence in wheat and Arabidopsis, and affected several agricultural traits in transgenic wheat. Moreover, impaired expression of TaNAC69-B by virus-induced gene silencing retarded the leaf senescence in wheat. By RNA-seq and quantitative real-time polymerase chain reaction analysis, we confirmed that some abscisic acid (ABA) biosynthesis genes, including AAO3 and its ortholog in wheat, TraesCS2B02G270600 (TaAO3-B), were elevated by the overexpression of TaNAC69-B. Consistently, we observed more severe ABA-induced leaf senescence in TaNAC69-B-OE wheat and Arabidopsis plants. Furthermore, we determined that TaNAC69-B bound to the NAC binding site core (CGT) on the promoter regions of AAO3 and TaAO3-B. Moreover, we confirmed elevated ABA levels in TaNAC69-B-OE wheat lines. Although TaNAC69-B shares 39.83% identity (amino acid) with AtNAP, TaNAC69-B did not completely restore the delayed leaf senescence in the atnap mutant. Collectively, our results revealed a positive feedback loop, consisting of TaNAC69-B, ABA biosynthesis and leaf senescence, that is essential for the regulation of leaf senescence in wheat.

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