Expression of genes encoding terpenoid biosynthesis enzymes during leaf development of Eucalyptus camaldulensis
文献类型： 外文期刊
作者： Zhan, N. 1 ; Huang, L. 3 ; Wang, Z. 4 ; Zhang, J. 1 ; Xie, Y. 1 ; Shang, X. 1 ; Liu, G. 1 ; Wu, Z. 1 ;
作者机构： 1.Chinese Acad Forestry, China Eucalypt Res Ctr, Guangzhou 524022, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Guangdong, Peoples R China
3.Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China
4.Beihai City Protect Forest Farm, Beihai 536000, Guangxi, Peoples R China
关键词： Eucalyptus camaldulensis; regulation mechanism; terpenoid biosynthesis; transcriptome; young and mature leaves
期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.122； 五年影响因子：1.923 ）
ISSN： 0006-3134
年卷期： 2022 年 66 卷
页码：
收录情况： SCI
摘要： To reveal the regulation mechanism of terpenoid biosynthesis in the leaves of Eucalyptus camaldulensis, the content of volatiles in eucalyptus leaves and the transcriptome databases of young and mature leaves were analyzed. The results showed that E. camaldulensis contains 92 and 89 kinds of volatile substances in the young and mature leaves, respectively. Among them, the content of 1,8-cineole, ??-pinene, and other substances was significantly different in young and mature leaves. A total of 99 802 unigenes were obtained from the transcriptome database of young and mature leaves of E. camaldulensis and 18 441 genes displayed obviously differential expressions during both developmental stages. There were 6 982 up-regulated unigenes and 11 461 down-regulated unigenes in the young leaf stage compared to the mature leaf stage. The key genes for terpenoid biosynthesis, including limonene synthase-10, limonene synthase-11, myrcene synthase-1, ??-pinene synthase-2, and 1,8-cineole synthase-2, were selected for further analysis to explore the mechanism of gene regulation and genetic transformation. The expressions of key genes were validated by RTqPCR, and their expressions were consistent with RNA-seq data. WRKY, MYB, NAC, and bHLH transcription factors (TFs) displayed important regulatory effects on the above key genes. Thus, a regulatory network model of terpenoid biosynthesis was constructed using target genes and TFs during leaf development in E. camaldulensis. These results provide theoretical evidence for understanding the terpenoid biosynthesis in plants and reference for terpenoids utilization by genetic engineering methods in E. camaldulensis.
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