文献类型： 外文期刊
作者： Wang, Xuchu 1 ; Tian, Weimin 2 ; Li, Yinxin 1 ;
作者机构： 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China
2.Chinese Acad Trop Agr Sci, Key Lab Minist Agr Trop Crops Physiol, Danzhou, Peoples R China
关键词： Lychee;Recalcitrant plant tissues;RNA extraction method;Trees
期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utilized EDTA, LSS, and CTAB to successfully inhibit RNase activities. It was found that a high ionic strength brought about by 2 M NaCl was necessary. In addition, secondary metabolites and other interfering compounds were effectively removed using sodium borate and PVPP under a deoxidized condition. The quality of purified RNA was tested by both RACE and Northern blotting analysis, ensuring that the RNA could be used for subsequent gene expression analysis. This method has been successfully applied to purify RNA from 15 other plant species. In conclusion, the protocol reported here is expected to have excellent applications for RNA isolation from recalcitrant plant tissues.
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Tianhan;Zhang, Yong E.;Liao, Xingyu;He, Shun-min;Chen, Runsheng;Yang, Fengchun;Li, Jiao;Liao, Xingyu;He, Shun-min;Chen, Runsheng;Yang, Fengchun;Li, Jiao;Liao, Xingyu;Zhang, Guoqing;Li, Yixue;Zhao, Guoping;Ling, Yunchao;Wang, Yiwei;Yang, Jiaxin;Zhuang, Xinhao;Liu, Wan;Cao, Ruifang;Zhang, Guo-qing;Zhou, Chenfen;Wang, Pengyu;Zhang, Guoqing;Li, Yixue;Zhao, Guoping;Ling, Yunchao;Wang, Yiwei;Yang, Jiaxin;Zhuang, Xinhao;Liu, Wan;Cao, Ruifang;Zhang, Guo-qing;Zhou, Chenfen;Wang, Pengyu;Zhao, Guoping;Zhao, Guoping;Chen, Runsheng;Zhang, Zaichao;Xia, Zhiqiang;Zhou, Xincheng;Chao, Jin-quan;Tian, Weimin;Huang, Yue;Xing, Peiqi;Liu, Zhaoqi;Pan, Siyu;Kang, Hongen;Liu, Xinxuan;Lin, Shiqi;Yuan, Na;Jia, Peilin;Xu, Xiaowei;Kang, Hongyu;Hou, Li;Gao, Qianwen;Liu, Jie;Wang, Jinyue;Liu, Xinxuan;Zhang, Yuanpu;Wang, Zhennan;Yuan, Hao;Su, Tianhan;Zhang, Yong E.;Wang, Zhennan;Yuan, Hao;Su, Tianhan;Zhang, Yong E.;Yao, Qiliang;Luo, Sicheng;Shen, Wen-Kang;Guo, An-Yuan;Liu, Dan;Zhang, Chi;Xue, Yu;Gou, Yujie;Chen, Miaomiao;Peng, Di;Ning, Wanshan;Wei, Yuxiang;Liu, Chun-Jie;Xie, Gui-Yan;Zhang, Qiong;Fu, Shanshan;Tan, Xiaodan;Tang, Dachao;Zhang, Weizhi;Luo, Mei;Miao, Ya-Ru;Huang, Xinhe;Feng, Zihao;Xie, Guiyan;Han, Cheng;Tang, Qing;Zuo, Zhixiang;Ren, Jian;Xie, Yubin;Luo, XiaoTong;Zhang, Xinxin;Xiao, Yun;Li, Xia;Zhao, Zheng;Jiang, Tao;Wu, Wanying;Zhao, Fangqing;Chen, Ming;Meng, Xianwen;Zhou, Yincong;Yuan, Chunhui;Wu, Wenyi;Chen, Ming;Meng, Xianwen;Zhou, Yincong;Yuan, Chunhui;Wu, Wenyi;Luo, Hao;Gao, Feng;Luo, Hao;Gao, Feng;Luo, Hao;Gao, Feng;Luo, Hao;Gao, Feng;Zhang, Yong E.;Guo, Guoji;Gao, Xin;Liao, Xingyu;Wang, Jianxin;Yang, Dechang;Tian, Feng;Gao, Ge;Yang, Dechang;Tian, Feng;Gao, Ge;Cui, Qinghua;Cui, Qinghua;Xiao, Chunfu;Li, Chuan-Yun;Liu, Pei;Sun, Yongqing;Sang, Jian
关键词：
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Functional Identification of ICE Transcription Factors in Rubber Tree
作者：Li, Yan;Quan, Caihong;Yang, Shuguang;Wu, Shaohua;Shi, Minjing;Wang, Jun;Tian, Weimin;Li, Yan;Quan, Caihong;Yang, Shuguang;Wu, Shaohua;Shi, Minjing;Wang, Jun;Tian, Weimin
关键词：Hevea brasiliensis Muell; Arg; ICE transcription factor; cold tolerance; real-time quantitative PCR; gene overexpression
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Genome-wide identification and expression analysis of the phosphatase 2A family in rubber tree (Hevea brasiliensis)
作者：Chao, Jinquan;Yang, Shuguang;Deng, Xiaomin;Tian, Weimin;Huang, Zhejun
关键词：
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Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex
作者：Yu, Li;Yuan, Boxuan;Xie, Quanliang;Wang, Xuchu;Yu, Li;Yuan, Boxuan;Wang, Lingling;Ding, Guohua;Souleymane, Ousmane Ahmat;Zhang, Xueyan;Wang, Xuchu;Sun, Yong
关键词：comparative proteomics; Hevea brasiliensis; Hydroxymethylglutaryl-CoA synthase; glycosylated proteins; natural rubber biosynthesis; rubber latex
