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Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)

文献类型: 外文期刊

作者: Shi, Yan 1 ; Zhu, Xin-Ping 1 ; Yin, Jing-Kui 1 ; Zhang, Qi-Ya 2 ; Gui, Jian-Fang 2 ;

作者机构: 1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China

2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

3.Guangzhou Univ, Coll Life Sci, Guangzhou 510006, Guangdong, Peoples R China

关键词: apoptosis;immune response;cell growth regulation;antiviral defense

期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Interferon-regulatory factor 1 (IRF-1) is the first member of IRF family, which is involved in many biological processes such as immune response, antiviral defense, cell growth regulation, and apoptosis. In this study, an IRF-1 gene, EcIRF-1, was isolated and characterized from orange-spotted grouper (Epinephelus coioides). The full-length cDNA of EcIRF-1 is 1,730 bp, including an open reading frame of 906 bp, a 5o-terminal untranslated region (5o-UTR) of 153 bp, and a 3o-UTR of 671 bp. The EcIRF-1 gene consists of 10 exons and 9 introns, spanning over approximate 4.3 kb of genomic sequence. The 5o-UTR sequence contains an exon and an intron, and the 3o-UTR sequence is included in the last exon. Expression analysis by real-time PCR reveals that the EcIRF-1 gene is ubiquitously expressed in various healthy fish tissues, whereas its expression is upregulated in vivo in response to polyinosinic-polycytidylic acid or lipopolysaccharide stimulation. Subcellular localization analysis shows the EcIRF-1 is an intranuclearly localized and immobile protein in the cultured fish cells. Data presented in this paper provide an important base to further understand EcIRF-1 gene function and its regulation associated with interferon immune system in orange-spotted grouper.

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