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Pistil drip following pollination: a simple in planta Agrobacterium-mediated transformation in cotton

文献类型: 外文期刊

作者: Chen TianZi 1 ; Wu ShenJie 1 ; Zhao Jun 1 ; Guo WangZhen 1 ; Zhang TianZhen 1 ;

作者机构: 1.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

2.Shanxi Acad Agr Sci, Cotton Res Inst, Yuncheng 030006, Peoples R China

关键词: cotton;genetic engineering;genetic transformation;genomes;herbicide resistance;heritability;transgenic plants;Gossypium

期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Transgenic cotton plants were developed by pistil drip inoculation in a solution containing Agrobacterium carrying a gene for resistance to the herbicide Basta (bar), 10% (w/v) sucrose, 0.05% (v/v) Silwet L-77 and 40 mg acetosyringone lp#. Pistil drip during 17:00-19:00 on the first day of flowering resulted in 0.07-0.17% Basta-resistant plants/number of viable seeds generated, and stigma excision prior to pistil drip during this time period gave rise to a transformation efficiency of 0.46-0.93%, in contrast with 0.04-0.06% generated from pistil drip during 9:00-11:00 on the second day of flowering. PCR and Southern blot analysis confirmed the integration of the bar gene into the cotton genome, and a T1 and T2 generation herbicide resistance test consistently revealed expression and stable heritability of the bar gene in the two generations.

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