文献类型： 外文期刊
作者： Wang, Xiaoyu 1 ; Wang, Genquan 1 ;
作者机构： 1.Shanxi Acad Agr Sci, Inst Millet Res, Changzhi, Peoples R China
2.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Peoples R China
3.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China
关键词： Candidate genes; Segregation population; NGS-BSA; Modified QTL-Seq
期刊名称：JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：1.525； 五年影响因子：1.344 ）
ISSN： 0971-7811
年卷期：
页码：
收录情况： SCI
摘要： BSA (bulked-segregant analysis) can be defined as a rapid procedure for identifying genomic regions responsible for complex traits in crops. The method involves comparing two bulked DNA samples generated from a segregating population originating from a single cross. With the improvement of next-generation sequencing (NGS) technology, BSA combined with next-generation sequencing (NGS-BSA) has been widely applied to QTL mapping due to its high efficiency. Following a summary of widely used NGS-BSA methods, we present a novel strategy of NGS-BSA application, called as Modified QTL-seq. For Modified QTL-seq, QTL-seq analysis is performed on paired bulks,followed by multiple comparison analysis, which can significantly narrow the candidate intervals. Besides, F-4:5 progeny used for additional QTL-seq analysis are only polymorphic at the target locus, which diminishs the heterosis influence on construction of bulked DNA, thus further narrowing candidate regions. Therefore, Modified QTL-seq is another optimization of NGS-BSA application. Compared with previous BSA methods, Modified QTL-seq is potential and cost-effective, and will accelerate QTL-mapping in crops.
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