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Effect of Piper nigrum essential oil in dextran sulfate sodium (DSS)-induced colitis and its potential mechanisms

文献类型： 外文期刊

作者： Hu, Yeye 1 ; He, Ziliang 2 ; Zhang, Ji 3 ; Zhang, Chaohua 1 ; Wang, Yanting 2 ; Zhang, Wei 3 ; Zhang, Fenglun 4 ; Zhang, Weiming 4 ; Gu, Fenglin 1 ; Hu, Weicheng 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Sanya Res Inst, Hainan 572025, Peoples R China

2.Yangzhou Univ, Inst Translat Med, Sch Med, Yangzhou 225009, Peoples R China

3.Huaiyin Normal Univ, Sch Life Sci, Huaian 223300, Peoples R China

4.Nanjing Inst Comprehens Utilizat Wild Plants, Nanjing 211111, Peoples R China

5.Key Lab Proc Suitabil & Qual Control Special Trop, Wanning 571533, Hainan, Peoples R China

6.Yangzhou Univ, Sch Med, Jiangsu Key Lab Expt & Translat Noncoding RNA Res, Yangzhou 225009, Peoples R China

关键词： Piper nigrum essential oil; Colitis; Gut microbiome; Intestinal barrier

期刊名称：PHYTOMEDICINE （ 影响因子：7.9； 五年影响因子：6.8 ）

ISSN： 0944-7113

年卷期： 2023 年 119 卷

页码：

收录情况： SCI

摘要： Background: Piper nigrum essential oil (PnEO) possesses pleasant aroma, unique flavor, and various bioactivities; however, its role against colitis remains unclear. Purpose: In this study, we investigated the role of PnEO in relieving colitis and explored its potential mechanisms in a mouse model of dextran sulfate sodium (DSS)-induced colitis. Methods: Initially, we identified and quantified the components of PnEO by gas chromatography-mass spectrometry (GC-MS). Subsequently, we investigated the protective role of PnEO (50 and 200 mg/kg) in DSS-induced colitis in mice by evaluating disease activity index (DAI) scores and colon length, and performing histological analyses. Eyeball blood was collected and cytokines were determined using ELISA kits. The anti-inflammatory mechanisms of PnEO were analyzed by western blot (WB) and immunohistochemistry (IHC). The intestinal barrier function was evaluated according to tight junction (TJ) protein mRNA levels. We used 16S rRNA gene sequencing to analyze the intestinal microflora of mouse cecal contents. Results: Supplementation with PnEO (50 and 200 mg/kg) increased colon length and improved colon histopathology. PnEO regulated inflammatory responses by downregulating TLR4/MAPKs activation, thereby reducing the release of cytokines and mediators. Moreover, it also protected the intestinal barrier through enhancing the expression of claudin-1, claudin-3, occludin, ZO-1, and mucin 2. 16S rRNA gene sequencing revealed that PnEO (200 mg/kg) decreased the abundance of Akkermansia in the gut microbiome. Conclusion: PnEO treatment (50 and 200 mg/kg) relieved DSS-induced colitis by inhibiting TLR4/MAPK pathway and protecting intestinal barrier, and high-dose PnEO exhibited better effects. Moreover, PnEO (200 mg/kg) regulated key compositions of the gut microbiome, which indicated that it had therapeutic potential for sustaining gut health to lower the risk of colitis.

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