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Identification of SCAR markers linked to or, a gene inducing beta-carotene accumulation in Chinese cabbage

文献类型： 外文期刊

作者： Zhang, Fenglan 1 ; Wang, Guochen 1 ; Wang, Mei 1 ; Liu, Xiucun 1 ; Zhao, Xiuyun 1 ; Yu, Yangjun 1 ; Zhang, Deshuang 1 ; Y 1 ;

作者机构： 1.BAAFS, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China

关键词： Chinese cabbage;mutation;mutants;leaves;color;genes;chromosome mapping;random amplified polymorphic DNA technique;genetic markers

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

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收录情况： SCI

摘要： The or mutation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) is a recessive, single-locus mutation that causes the head leaves of the plant to accumulate carotenoids and turn orange. In China, considerable attention has been focused in recent years on breeding the variety with orange head leaves. In this study, sequence-characterized amplified region (SCAR) markers linked to the or gene were identified based on random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) by performing a bulked segregant analysis (BSA) using a doubled haploid (DH) population derived from the F cross between 91-112 (white head leaves) and T12-19 (orange head leaves) via microspore culture. Two RAPD markers--OPB01-845 and OPAX18-656--and 1 AFLP marker, namely, P67M54-172, were identified to be linked to the or gene, and they were successfully converted into the SCAR markers SCR-845, SCOR204, and SCOR127, respectively. In a linkage analysis, these 3 SCAR markers and 2 previously published simple sequence repeat markers, namely, BRMS-51 and Ni4D09 (located on R9 linkage group), were mapped to the same linkage group with the or gene at a LOD score of 6.0, indicating that the or gene should be located on the linkage group R9 of the A genome. In addition, accuracies of 92%, 90%, and 89.1% were obtained when 110 different inbred breeding lines of Chinese cabbage were used for investigation with these 3 SCAR markers, indicating that these makers could be used in marker-assisted selection in orange head leaf breeding programs for Chinese cabbage.

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